Trent University Graduate Thesis Collection

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Identifier
tula:etd
Type
Collection
Language
eng
Extent
1 item
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Copyright for all items in the Trent University Graduate Thesis Collection is held by the author, with all rights reserved, unless otherwise noted.
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An in vitro characterization of the novel binding properties of the Giardia TATA-binding protein

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Year: 2025, 2025
Member of: Trent University Graduate Thesis Collection
Name(s): Creator (cre): Freitag, Kieran, Thesis advisor (ths): Yee, Janet, Degree committee member (dgc): Brunetti, Craig, Degree committee member (dgc): Huber, Robert, Degree granting institution (dgg): Trent University
Abstract: <p>The TATA-binding protein (TBP) is a key regulator of eukaryotic transcription initiation. The TBP homolog from Giardia intestinalis (gTBP) is highly divergent among all TBPs; notably lacking three of the four phenylalanine residues to unwind double- stranded DNA. I show that gTBP preferentially binds to single-stranded DNA (ssDNA) in two modes based on sequence and protein concentration… more