Trent University Graduate Thesis Collection

Item Description
Identifier
tula:etd
Type
Collection
Language
eng
Extent
1 item
Rights
Copyright for all items in the Trent University Graduate Thesis Collection is held by the author, with all rights reserved, unless otherwise noted.
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Mutation of the B10 Tyrosine and E11 Leucine in Giardia intestinalis Flavohemoglobin

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Year: 2016, 2016
Member of: Trent University Graduate Thesis Collection
Name(s): Creator (cre): Lukaszewicz, Brian, Thesis advisor (ths): Rafferty, Steven P, Degree committee member (dgc): Saville, Barry J, Degree committee member (dgc): Yee, Janet, Degree granting institution (dgg): Trent University
Abstract: <p>The flavohemoglobin in Giardia intestinalis (gFlHb) is the only known protozoan member of a protein class typically associated with detoxifying nitric oxide (by oxidation to nitrate) in bacteria and yeast. Mutants of the B10 tyrosine (Y30F) and E11 leucine (L58A), conserved residues thought to influence ligand binding, were expressed and studied using Resonance Raman (RR) spectroscopy.… more

Electrochemical Characterization of Giardia Intestinalis Cytochromes b5

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Year: 2016, 2016
Member of: Trent University Graduate Thesis Collection
Name(s): Creator (cre): Yang, Zhen (Alice), Thesis advisor (ths): Rafferty, Steven P, Degree committee member (dgc): Vreugdenhil, Andrew J, Degree committee member (dgc): Saville, Barry J, Degree granting institution (dgg): Trent University
Abstract: <p>Giardia intestinalis is a protozoan parasite that causes waterborne diarrheal disease in animals and humans. It is an unusual eukaryote as it lacks the capacity for heme biosynthesis; nonetheless it encodes heme proteins, including three cytochrome b5 isotypes (gCYTB5s) of similar size. Homology modelling of their structures predicts increased heme pocket polarity compared to mammalian… more

Characterisation of the Giardia Tata-Binding Protein - Preparation for an in vivo approach

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Year: 2016, 2016
Member of: Trent University Graduate Thesis Collection
Name(s): Creator (cre): Kemp, Adam, Thesis advisor (ths): Yee, Janet, Degree committee member (dgc): Saville, Barry J, Degree committee member (dgc): Brunetti, Craig R, Degree granting institution (dgg): Trent University
Abstract: <p>The aim of this work was to identify the DNA sequences recognized by the Giardia TBP (gTBP) in vivo by using a chromatin immunoprecipitation assay (ChIP). Since a specific antibody for the protein of interest is required for this assay, a company was contracted to produce and purify a custom polyclonal antibody from the immunization of rabbits. Recombinant GST-gTBP was produced at a… more

Fungi and Cytokinins: Investigating the impact of cytokinins on fungal development and disease progression in the Ustilago maydis- Zea mays pathosystem

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Year: 2016, 2016
Member of: Trent University Graduate Thesis Collection
Name(s): Creator (cre): Morrison, Erin Nicole, Thesis advisor (ths): Emery, R.J.Neil, Thesis advisor (ths): Saville, Barry J, Degree committee member (dgc): Sutcliffe, Jim, Degree granting institution (dgg): Trent University
Abstract: <p>Cytokinin biosynthesis in organisms aside from plant species has often been viewed as a byproduct of tRNA degradation. Recent evidence suggests that these tRNA degradation products may actually have a role in the development of these organisms, particularly fungi. This thesis examines the importance of cytokinins, a group of phytohormones involved in plant cell division and… more