Year: 2016, 2016
Member of: Trent University Graduate Thesis Collection
Abstract: <p>Cytokinin biosynthesis in organisms aside from plant species has often been viewed as a byproduct of tRNA degradation. Recent evidence suggests that these tRNA degradation products may actually have a role in the development of these organisms, particularly fungi. This thesis examines the importance of cytokinins, a group of phytohormones involved in plant cell division and… more Full Text: FUNGI AND CYTOKININS: INVESTIGATING THE IMPACT OF CYTOKININS ON FUNGAL DEVELOPMENT AND DISEASE PROGRESSION IN THE Ustilago maydis- Zea mays PATHOSYSTEM A dissertation submitted to the Committee of Graduate Studies in partial fulfillment of the …
Year: 2016, 2016
Member of: Trent University Graduate Thesis Collection
Abstract: <p>The aim of this work was to identify the DNA sequences recognized by the Giardia TBP (gTBP) in vivo by using a chromatin immunoprecipitation assay (ChIP). Since a specific antibody for the protein of interest is required for this assay, a company was contracted to produce and purify a custom polyclonal antibody from the immunization of rabbits. Recombinant GST-gTBP was produced at a… more Full Text: CHARACTERISATION OF THE GIARDIA TATABINDING PROTEIN - PREPARATION FOR AN IN VIVO APPROACH A Thesis Submitted to the Committee of Graduate Studies in Partial Fulfilment of the Requirements for the degree of Master of Science in the Faculty of Arts and …
Year: 2016, 2016
Member of: Trent University Graduate Thesis Collection
Abstract: <p>The flavohemoglobin in Giardia intestinalis (gFlHb) is the only known protozoan member of a protein class typically associated with detoxifying nitric oxide (by oxidation to nitrate) in bacteria and yeast. Mutants of the B10 tyrosine (Y30F) and E11 leucine (L58A), conserved residues thought to influence ligand binding, were expressed and studied using Resonance Raman (RR) spectroscopy.… more Full Text: Mutation of the B10 Tyrosine and E11 Leucine in Giardia intestinalis Flavohemoglobin A thesis submitted to the Committee of Graduate Studies in partial fulfillment of the requirements for the degree of Master of Science in the Faculty of Arts and Science …
Year: 2016, 2016
Member of: Trent University Graduate Thesis Collection
Abstract: <p>Giardia intestinalis is a protozoan parasite that causes waterborne diarrheal disease in animals and humans. It is an unusual eukaryote as it lacks the capacity for heme biosynthesis; nonetheless it encodes heme proteins, including three cytochrome b5 isotypes (gCYTB5s) of similar size. Homology modelling of their structures predicts increased heme pocket polarity compared to mammalian… more Full Text: ELECTROCHEMICAL CHARACTERIZATION OF GIARDIA INTESTINALIS CYTOCHROMES b5 A Thesis Submitted to the Committee on Graduate Studies in Partial Fulfillment of the Requirements for the Degree of Masters of Science in the Faculty of Arts and Science TRENT …