Trent University Graduate Thesis Collection

Item Description
Identifier
tula:etd
Type
Collection
Language
eng
Extent
1 item
Rights
Copyright for all items in the Trent University Graduate Thesis Collection is held by the author, with all rights reserved, unless otherwise noted.
Displaying 1 - 2 of 2

Results per page

Displaying 1 - 2 of 2

Ligand Binding Properties of Giardia Flavohemoglobin

22492-Thumbnail Image.png
Year: 2019, 2019
Member of: Trent University Graduate Thesis Collection
Name(s): Creator (cre): Nezamololama, Novin, Thesis advisor (ths): Rafferty, Steven P, Degree committee member (dgc): Vreugdenhil, Andrew, Degree committee member (dgc): Bates, Holly, Degree granting institution (dgg): Trent University
Abstract: <p>The parasitic protist Giardia intestinalis possesses flavohemoglobin (gFlHb), an enzyme that detoxifies nitric oxide to the less harmful nitrate, and is a potential target for antigiardial drugs that act as ligands to the iron of its heme cofactor. In this work, the binding constants KD of gFlHb, three active-site variants (Q54L, L58A, Y30F) and the E. coli flavohemoglobin (Hmp) towards… more

The Effect of Nitrosative Stress on Heme Protein Expression and Localization in Giardia Intestinalis

22578-Thumbnail Image.png
Year: 2019, 2019
Member of: Trent University Graduate Thesis Collection
Name(s): Creator (cre): Sajer, Bayan, Thesis advisor (ths): Yee, Janet, Degree committee member (dgc): Rafferty, Steven, Degree committee member (dgc): Bates, Holly, Degree committee member (dgc): Brunetti, Craig, Degree granting institution (dgg): Trent University
Abstract: <p>The parasitic protist Giardia intestinalis has five heme proteins: a flavohemoglobin and several isotypes of cytochrome b5. While the flavohemoglobin has a role in counteracting nitric oxide, the functions of the cytochromes (gCYTb5s) are unknown. In this study, the protein level and cellular localization of three gCYTB5 isotypes (gCYTb5-I, II and III) and flavohemoglobin were examined… more