Year: 2019, 2019
Member of: Trent University Graduate Thesis Collection
Abstract: <p>The parasitic protist Giardia intestinalis possesses flavohemoglobin (gFlHb), an enzyme that detoxifies nitric oxide to the less harmful nitrate, and is a potential target for antigiardial drugs that act as ligands to the iron of its heme cofactor. In this work, the binding constants KD of gFlHb, three active-site variants (Q54L, L58A, Y30F) and the E. coli flavohemoglobin (Hmp) towards… more Full Text: Ligand Binding Properties of Giardia Flavohemoglobin A thesis submitted to the Committee of Graduate Studies in partial fulfillment of the requirements for the degree of Master of Science in the Faculty of Arts and Science Trent University Peterborough, …
Year: 2019, 2019
Member of: Trent University Graduate Thesis Collection
Name(s): Creator (cre): Sajer, Bayan, Thesis advisor (ths): Yee, Janet, Degree committee member (dgc): Rafferty, Steven, Degree committee member (dgc): Bates, Holly, Degree committee member (dgc): Brunetti, Craig, Degree granting institution (dgg): Trent University Abstract: <p>The parasitic protist Giardia intestinalis has five heme proteins: a flavohemoglobin and several isotypes of cytochrome b5. While the flavohemoglobin has a role in counteracting nitric oxide, the functions of the cytochromes (gCYTb5s) are unknown. In this study, the protein level and cellular localization of three gCYTB5 isotypes (gCYTb5-I, II and III) and flavohemoglobin were examined… more Full Text: THE EFFECT OF NITROSATIVE STRESS ON HEME PROTEIN EXPRESSION AND LOCALIZATION IN GIARDIA INTESTINALIS A Thesis Submitted to the Committee on Graduate Studies in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy in the Faculty …
