Bates, Holly

DNAJC5, an HSP40 member, supports synaptic vesicle release and protein folding byactivating HSP70 ATPase activity. In humans, it localizes to presynaptic terminals and endomembrane compartments that are involved in protein trafficking. Mutations in DNAJC5 cause CLN4 disease, a rare adult-onset Batten disease. Dictyostelium discoideum, a model for neurodegenerative research, encodes a putative homolog of DNAJC5, Dnajc5 (DDB0306688), which remains uncharacterized. This study examined Dnajc5 localization and function in D. discodieum. Dnajc5 localized to the endoplasmic reticulum, cytoplasm and nucleolus under both growth and starvation conditions, suggesting a role in proteostasis. Unlike human DNAJC5, Dnajc5 was absent from endomembrane compartments and extracellularly during starvation. Protein quantification revealed increased levels during early development, peaking at the mound stage, and declining thereafter—paralleling gene expression. Immunoprecipitation of Dnajc5 showed no serine phosphorylation or ubiquitination, unlike human DNAJC5. These findings suggest functional differences despite a possible common role in proteostasis.

Author Keywords: actinomycin- D, CLN4, Dictyostelium discoiduem, DNAJC5, Immunoprecipitation, multicellular development

The parasitic protist Giardia intestinalis has five heme proteins: a flavohemoglobin and several isotypes of cytochrome b5. While the flavohemoglobin has a role in counteracting nitric oxide, the functions of the cytochromes (gCYTb5s) are unknown. In this study, the protein level and cellular localization of three gCYTB5 isotypes (gCYTb5-I, II and III) and flavohemoglobin were examined in Giardia trophozoites exposed to three nitrosative stressors at two different concentrations: nitrite (20 mM, 0.5 mM); GSNO (2 mM, 0.25 mM) and DETA-NONOate (2 mM, 0.05 mM). An increase in protein levels was observed for gCYTb5-II with all stressors at both concentrations. However, the effects of these nitrosative stressors on gCYTb5-I and III were inconclusive due to the variation among the replicates and the poor detection of gCYTb5- III on western blots. The protein level of the flavohemoglobin also increased in response to the three stressors at the low concentrations of stressors that were tested. Only the cellular localization of gCYTb5-I changed in response to nitrosative stress, where it moved from the nucleolus to the nucleus and cytoplasm. This response was extremely sensitive and occurred at the lower doses of the three stressors, suggesting that gCYTb5-I may be involved in a nucleolar- based stress response.

The parasitic protist Giardia intestinalis possesses flavohemoglobin (gFlHb), an enzyme that detoxifies nitric oxide to the less harmful nitrate, and is a potential target for antigiardial drugs that act as ligands to the iron of its heme cofactor. In this work, the binding constants KD of gFlHb, three active-site variants (Q54L, L58A, Y30F) and the E. coli flavohemoglobin (Hmp) towards cyanide, azide and several substituted imidazoles were measured by optical titration. Certain cases such as gFlHb and Hmp were studied further by isothermal titration calorimetry. Binding constants for cyanide and the imidazoles ranged from 2 to 100 M, with the highest affinities observed with for miconazole, a bulky substituted imidazole. Azide was a poor ligand, with binding constants between 0.48 and 26 mM. Among gFlHb and its mutants, L58A tended to have the highest ligand affinities, as mutation of the distal leucine to a less bulky distal alanine residue facilitates the access of the exogenous ligand to the heme iron. In contrast, the Q54L and Y30F variants had binding affinities that in most cases were similar to wild type, which suggests that the inability of their side chains to form hydrogen bonds to these ligands is not a significant factor in binding of imidazole ligands to the enzyme. Comparative results for Hmp and gFlHb ligand binding affinities revealed slight differences which might be explained by the presence of different residues in their active sites apart from their conserved residues.

Author Keywords: Flavohemoglobin, Giardia intestinalis, Imidazole binding, Ligand binding, Nitrosative stress

The length of photoperiod can alter circadian rhythms and change fat depot mass whencombined with environmental temperatures below thermoneutral. To isolate photoperiod effects, we compared the effects of long and short photoperiod exposure at thermoneutrality in photoperiod sensitive, F1 generation adult male white-footed mice (P. leucopus). Mice were housed in long-day or short-day photoperiod conditions at thermoneutrality for 4 weeks. Short photoperiod decreased vWAT mass without changing body weight, scWAT or iBAT mass, or calorie consumption. Short photoperiod increased Adrβ3 and Lpl mRNA expression in vWAT with no change in Ucp1, Pgc1a or Hsl. vWAT Per1, Per2 and Nr1d1 mRNA expression were aligned to the onset of dark and food intake, while Bmal1 and Clock were misaligned. These findings suggest that short photoperiod per se can decrease visceral fat accumulation, without activating thermogenesis, reinforcing that environmental photoperiod should be considered when researching cause and prevention of obesity.

Author Keywords: adiposity, circadian rhythm, clock genes, obesity, Peromyscus, photoperiod