Trent University Graduate Thesis Collection

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Identifier

tula:etd

Type

Collection

Language

eng

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1 item

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Displaying 1 - 2 of 2

Expression optimization and NMR spectroscopy of Giardia intestinalis cytochrome b5 isotype III

Year: 2019, 2019

Member of: Trent University Graduate Thesis Collection

Name(s): Creator (cre): Crowley, Erika Lynn, Thesis advisor (ths): Rafferty, Steven P, Degree committee member (dgc): Vreugdenhil, Andrew, Degree committee member (dgc): Brunetti, Craig, Degree granting institution (dgg): Trent University

Abstract: <p>The parasitic protist Giardia intestinalis does not synthesize heme and lacks many common eukaryotic heme proteins, yet it expresses four cytochrome b5 (gCYTB5) isotypes of unknown function. These have low reduction potentials and distinct subcellular locations that are consistent with structural features and biological functions that differ from their mammalian counterparts. Isotype III… more

The Effect of Nitrosative Stress on Heme Protein Expression and Localization in Giardia Intestinalis

Year: 2019, 2019

Member of: Trent University Graduate Thesis Collection

Name(s): Creator (cre): Sajer, Bayan, Thesis advisor (ths): Yee, Janet, Degree committee member (dgc): Rafferty, Steven, Degree committee member (dgc): Bates, Holly, Degree committee member (dgc): Brunetti, Craig, Degree granting institution (dgg): Trent University

Abstract: <p>The parasitic protist Giardia intestinalis has five heme proteins: a flavohemoglobin and several isotypes of cytochrome b5. While the flavohemoglobin has a role in counteracting nitric oxide, the functions of the cytochromes (gCYTb5s) are unknown. In this study, the protein level and cellular localization of three gCYTB5 isotypes (gCYTb5-I, II and III) and flavohemoglobin were examined… more