Year: 2019, 2019
Member of: Trent University Graduate Thesis Collection
Abstract: <p>The parasitic protist Giardia intestinalis does not synthesize heme and lacks many common eukaryotic heme proteins, yet it expresses four cytochrome b5 (gCYTB5) isotypes of unknown function. These have low reduction potentials and distinct subcellular locations that are consistent with structural features and biological functions that differ from their mammalian counterparts. Isotype III… more Full Text: Expression optimization and NMR spectroscopy of Giardia intestinalis cytochrome b5 isotype III A thesis submitted to the Committee on Graduate Studies in partial fulfillment of the requirements for the degree of Master of Science in the Faculty of Arts …
Year: 2019, 2019
Member of: Trent University Graduate Thesis Collection
Abstract: <p>The parasitic protist Giardia intestinalis possesses flavohemoglobin (gFlHb), an enzyme that detoxifies nitric oxide to the less harmful nitrate, and is a potential target for antigiardial drugs that act as ligands to the iron of its heme cofactor. In this work, the binding constants KD of gFlHb, three active-site variants (Q54L, L58A, Y30F) and the E. coli flavohemoglobin (Hmp) towards… more Full Text: Ligand Binding Properties of Giardia Flavohemoglobin A thesis submitted to the Committee of Graduate Studies in partial fulfillment of the requirements for the degree of Master of Science in the Faculty of Arts and Science Trent University Peterborough, …
Year: 2017, 2017
Member of: Trent University Graduate Thesis Collection
Abstract: <p>The amitochondrial protozoan, Giardia intestinalis, encodes four members of the cytochrome b5 (CYTB5) family of heme proteins of unknown function. While homology models can predict the likely fold of these proteins, supporting experimental evidence is lacking. The small size of the cytochromes (~16 kDa) makes them attractive targets for structural analysis by Electron Paramagnetic… more Full Text: NMR and EPR Studies on Cytochrome b5 Isotypes of Giardia intestinalis A thesis submitted to the Committee on Graduate Studies in Partial Fulfillment of the requirements for the degree of Master of Science in the Faculty of Arts and Science Trent …
Year: 2017, 2017
Member of: Trent University Graduate Thesis Collection
Abstract: <p>Chemical cues are used commonly by prey to identify predation risk in aquatic environments. Previous work has indicated that negatively-charged ions of m/z 501 are possibly a kairomone that induces anti-predator responses in tadpoles. This thesis found that this ion species: (i) is produced by injured tadpoles; (ii) exhibits increased spectral intensity with higher tadpole biomass; and (… more Full Text: THE ORIGIN AND ECOLOGICAL FUNCTION OF AN ION INDUCING ANTIPREDATOR BEHAVIOUR IN LITHOBATES TADPOLES A Thesis Submitted to the Committee on Graduate Studies in Partial Fulfillment of the Requirements for the Degree of Master of Science in the Faculty of …
Year: 2016, 2016
Member of: Trent University Graduate Thesis Collection
Abstract: <p>The flavohemoglobin in Giardia intestinalis (gFlHb) is the only known protozoan member of a protein class typically associated with detoxifying nitric oxide (by oxidation to nitrate) in bacteria and yeast. Mutants of the B10 tyrosine (Y30F) and E11 leucine (L58A), conserved residues thought to influence ligand binding, were expressed and studied using Resonance Raman (RR) spectroscopy.… more Full Text: Mutation of the B10 Tyrosine and E11 Leucine in Giardia intestinalis Flavohemoglobin A thesis submitted to the Committee of Graduate Studies in partial fulfillment of the requirements for the degree of Master of Science in the Faculty of Arts and Science …
Year: 2016, 2016
Member of: Trent University Graduate Thesis Collection
Abstract: <p>Giardia intestinalis is a protozoan parasite that causes waterborne diarrheal disease in animals and humans. It is an unusual eukaryote as it lacks the capacity for heme biosynthesis; nonetheless it encodes heme proteins, including three cytochrome b5 isotypes (gCYTB5s) of similar size. Homology modelling of their structures predicts increased heme pocket polarity compared to mammalian… more Full Text: ELECTROCHEMICAL CHARACTERIZATION OF GIARDIA INTESTINALIS CYTOCHROMES b5 A Thesis Submitted to the Committee on Graduate Studies in Partial Fulfillment of the Requirements for the Degree of Masters of Science in the Faculty of Arts and Science TRENT …
Year: 2015, 2015
Member of: Trent University Graduate Thesis Collection
Abstract: <p>Giardia lamblia is an intestinal parasite found globally in freshwater systems that is responsible for endemic outbreaks of infectious diarrhea. As a unicellular parasite that lacks mitochondria, a respiratory chain and lives in the anaerobic environment of its host's intestine, Giardia was assumed for decades to lack heme proteins. However, its genome encodes several putative heme… more Full Text: i Expression and characterization of cytochrome b5 from Giardia lamblia A thesis submitted to the Committee of Graduate Studies in partial fulfillment of the requirements for the degree of Master of Science in the Faculty of Arts and Science Trent …
