Trent University Graduate Thesis Collection

Item Description
Identifier
tula:etd
Type
Collection
Language
eng
Extent
1 item
Rights
Copyright for all items in the Trent University Graduate Thesis Collection is held by the author, with all rights reserved, unless otherwise noted.
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Ligand Binding Properties of Giardia Flavohemoglobin

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Year: 2019, 2019
Member of: Trent University Graduate Thesis Collection
Name(s): Creator (cre): Nezamololama, Novin, Thesis advisor (ths): Rafferty, Steven P, Degree committee member (dgc): Vreugdenhil, Andrew, Degree committee member (dgc): Bates, Holly, Degree granting institution (dgg): Trent University
Abstract: <p>The parasitic protist Giardia intestinalis possesses flavohemoglobin (gFlHb), an enzyme that detoxifies nitric oxide to the less harmful nitrate, and is a potential target for antigiardial drugs that act as ligands to the iron of its heme cofactor. In this work, the binding constants KD of gFlHb, three active-site variants (Q54L, L58A, Y30F) and the E. coli flavohemoglobin (Hmp) towards… more

Expression optimization and NMR spectroscopy of Giardia intestinalis cytochrome b5 isotype III

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Year: 2019, 2019
Member of: Trent University Graduate Thesis Collection
Name(s): Creator (cre): Crowley, Erika Lynn, Thesis advisor (ths): Rafferty, Steven P, Degree committee member (dgc): Vreugdenhil, Andrew, Degree committee member (dgc): Brunetti, Craig, Degree granting institution (dgg): Trent University
Abstract: <p>The parasitic protist Giardia intestinalis does not synthesize heme and lacks many common eukaryotic heme proteins, yet it expresses four cytochrome b5 (gCYTB5) isotypes of unknown function. These have low reduction potentials and distinct subcellular locations that are consistent with structural features and biological functions that differ from their mammalian counterparts. Isotype III… more