The growth of immature insects that develop on human remains can be used to estimate a post-mortem interval (PMI). PMI estimate confidence is negatively affected by: larval killing and preservation methods altering their size, limited morphological parameters to assess larval growth and therefore age, and few available alternate species development data. I compared live specimens to preserved specimens of the same development stages to assess the effects of killing-preservation techniques on morphology, and I introduce a new method that uses digital photography to examine maggot mouthparts for stage grading of Phormia regina. Digital photographic methods enable live insects to be quantified and improve approximations of physiological age. I then use these digital methods to produce a growth-rate model for a beetle commonly found on human remains, Necrodes surinamensis, providing data for PMI estimates that was previously unavailable.
Author Keywords: Forensic Entomology, Insect development, Morphometrics, Necrodes surinamensis, Phormia regina, Postmortem interval