Year: 2025, 2025
Member of: Trent University Graduate Thesis Collection
Abstract: <p>DNAJC5, an HSP40 member, supports synaptic vesicle release and protein folding byactivating HSP70 ATPase activity. In humans, it localizes to presynaptic terminals and
endomembrane compartments that are involved in protein trafficking. Mutations in DNAJC5
cause CLN4 disease, a rare adult-onset Batten disease. Dictyostelium discoideum, a model for
neurodegenerative research, encodes a… more Year: 2025, 2025
Member of: Trent University Graduate Thesis Collection
Abstract: <p>The TATA-binding protein (TBP) is a key regulator of eukaryotic transcription initiation. The TBP homolog from Giardia intestinalis (gTBP) is highly divergent among all TBPs; notably lacking three of the four phenylalanine residues to unwind double- stranded DNA. I show that gTBP preferentially binds to single-stranded DNA (ssDNA) in two modes based on sequence and protein concentration… more Year: 2024, 2024
Member of: Trent University Graduate Thesis Collection
Name(s): Creator (cre): Knight, Caitlyn, Thesis advisor (ths): Kapron, Carolyn, Degree committee member (dgc): Tobin, Stephanie, Degree committee member (dgc): Huber, Robert, Degree committee member (dgc): West, Sarah, Degree granting institution (dgg): Trent University Abstract: <p>Cadmium is a toxic metal that has detrimental effects on blood vessel development and function. To examine the effect of varying concentrations of cadmium on angiogenesis, two in vitro assays were used. First, developing yolk sac blood vessels were studied in gestation day 8 mouse embryos exposed to medium alone, 1.25, or 1.75 μM cadmium chloride (CdCl2). Embryos exposed to 1.25 μM… more Full Text: Concentration-Dependent Effects of Cadmium on Mouse Angiogenesis In Vitro A Thesis Submitted to the Committee on Graduate Studies in Partial Fulfillment of the Requirements for the Degree of Science in the Faculty of Arts and Science TRENT UNIVERSITY …
Year: 2024, 2024
Member of: Trent University Graduate Thesis Collection
Name(s): Creator (cre): Knight, Caitlyn, Thesis advisor (ths): Kapron, Carolyn, Degree committee member (dgc): Tobin, Stephanie, Degree committee member (dgc): Huber, Robert, Degree committee member (dgc): West, Sarah, Degree granting institution (dgg): Trent University Abstract: <p>Cadmium is a toxic metal that has detrimental effects on blood vessel development and function. To examine the effect of varying concentrations of cadmium on angiogenesis, two in vitro assays were used. First, developing yolk sac blood vessels were studied in gestation day 8 mouse embryos exposed to medium alone, 1.25, or 1.75 μM cadmium chloride (CdCl2). Embryos exposed to 1.25 μM… more Year: 2023, 2023
Member of: Trent University Graduate Thesis Collection
Abstract: <p>Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disorder characterized by progressive loss in function of motor neurons. Elevated levels of biologically important metal ions, such as copper (II) (Cu(II)), zinc (II) (Zn(II)) or iron (III) (Fe(III)), may contribute to the disease. Moreover, Cu(II) interactions with other proteins associated with ALS have been investigated;… more Full Text: ELECTROCHEMICAL AND SURFACE ANALYSIS OF METAL IONS AND TDP-43 PROTEIN INTERACTIONS A Thesis Submitted to the Committee of Graduate Studies in Partial Fulfillment of the Requirements for the Degree of Master of Science in the Faculty of Arts and Science …
Year: 2021, 2021
Member of: Trent University Graduate Thesis Collection
Abstract: <p>The emergence of fungal hybrid pathogens threatens sustainable crop production worldwide. To investigate hybridization, the related smut fungi, Ustilago maydis and Sporisorium reilianum, were selected because they infect a common host (Zea mays), can hybridize, and tools are available for their analysis. Hybrid dikaryons exhibited filamentous growth on plates but reduced virulence and… more Full Text: Fungal pathogen emergence: an Ustilago maydis x Sporisorium reilianum model A thesis submitted to the Committee of Graduate Studies in partial fulfillment of the requirements for the degree of Master of Science in the faculty of Arts and Science TRENT …
Year: 2021, 2021
Member of: Trent University Graduate Thesis Collection
Abstract: <p>Transactive response (TAR) DNA-binding protein of 43 kDa (TDP-43) pathology, including fibrillar aggregates and mutations, develops in amyotrophic lateral sclerosis (ALS) and is characterized by hyperphosphorylation and aggregation patterns, a mechanism largely understudied. In addition, ALS remains without a cure. Herein, in vitro aggregation of phosphorylated TDP-43 was explored, and… more Full Text: IMMUNOTHERAPIES TARGETING THE AMYOTROPHIC LATERAL SCLEROSIS-ASSOCIATED PROTEIN TDP-43 A Thesis Submitted to the Committee of Graduate Studies in Partial Fulfillment of the Requirements for the Degree of Master of Science in the Faculty of Arts and …
Year: 2021, 2021
Member of: Trent University Graduate Thesis Collection
Abstract: <p>Giardia intestinalis possesses four isotypes of cytochrome b5 (gCYTB-I-IV) that differ from their mammalian counterparts, suggesting different functions in this protozoan parasite. Although the recently discovered Giardia flavoenzyme, GiOR-1, reduces these cytochromes, its properties have not been thoroughly studied, owing to the difficulty in its expression. Here I describe successful… more Full Text: Expression of Giardia intestinalis flavoenzyme GiOR-1 and characterization of its electron transfer properties A thesis submitted to the Committee on Graduate Studies in partial fulfillment of the requirements for the degree of Master of Science in the …
Year: 2020, 2020
Member of: Trent University Graduate Thesis Collection
Abstract: <p>The waterborne protozoan Giardia intestinalis cycles between the environmentally-resistant and infectious cyst and the metabolically-active trophozoite that adheres to the epithelial lining of the small intestine. Adhesion can trigger the innate immune response in epithelial cells, including the synthesis of the free radical nitric oxide (NO) that inhibits cell proliferation and… more Full Text: Differential expression of cytochrome b5 proteins in Giardia intestinalis during nitrosative stress and encystation TRENT UNIVERSITY Peterborough, Ontario, Canada © Copyright by Kaitlyn Mowat 2020 Environmental and Life Sciences M. Sc. Graduate Program …
