Trent University Graduate Thesis Collection

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    Copyright for all items in the Trent University Graduate Thesis Collection is held by the author, with all rights reserved, unless otherwise noted.
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    The search for myco-hormones in the metabolome of the model organism Sordaria macrospora via LC-MS/MS.

    Year: 2024, 2024
    Member of: Trent University Graduate Thesis Collection
    Name(s): Creator (cre): Molina Bean, Kimberly, Thesis advisor (ths): Emery, Neil, Degree committee member (dgc): Kisiala, Anna, Degree committee member (dgc): Morrison, Erin, Degree granting institution (dgg): Trent University
    Abstract: <p>Fungi are a diverse group of organisms that play crucial roles in various ecological processes and have immense economic importance. Understanding the intricate mechanisms underlying fungal growth and development is fundamental to harnessing their potential and exploring their applications in different fields. Signalling molecules, such as hormones, have been identified as key regulators… more

    Impacts of Cover Crops on Soil Health, Soil Nitrogen Dynamics, and Cytokinin Profiles

    Year: 2023, 2023
    Member of: Trent University Graduate Thesis Collection
    Name(s): Creator (cre): Perera, Ushettige Imesha Dimuthuni, Thesis advisor (ths): Thompson, Karen A, Thesis advisor (ths): Emery, Neil, Degree committee member (dgc): Kisiala, Anna, Degree granting institution (dgg): Trent University
    Abstract: <p>In Ontario, the dominant cash crop rotations consist of soybean (SB), which is a leguminous crop grown in rotation with maize (MZ) and winter wheat (WW). In addition to these crops, some farmers integrate cover crops (CC) into crop rotation, especially during the fallow period and winter seasons, to reduce nitrogen (N) losses via nitrate (NO3-) leaching and emission of N2 and the… more

    Electrochemical and Surface Analysis of Metal Ions and TDP-43 Protein Interactions

    Year: 2023, 2023
    Member of: Trent University Graduate Thesis Collection
    Name(s): Creator (cre): Tabobondung, Meaghan O. F., Thesis advisor (ths): Martic, Sanela, Degree committee member (dgc): Emery, Neil, Degree committee member (dgc): Huber, Robert, Degree granting institution (dgg): Trent University
    Abstract: <p>Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disorder characterized by progressive loss in function of motor neurons. Elevated levels of biologically important metal ions, such as copper (II) (Cu(II)), zinc (II) (Zn(II)) or iron (III) (Fe(III)), may contribute to the disease. Moreover, Cu(II) interactions with other proteins associated with ALS have been investigated;… more

    Clonal structure and mating patterns in a natural population of Sagittaria latifolia

    Year: 2022, 2022
    Member of: Trent University Graduate Thesis Collection
    Name(s): Creator (cre): Stephens, Samantha, Thesis advisor (ths): Dorken, Marcel, Degree committee member (dgc): Emery, Neil, Degree committee member (dgc): Bowman, Jeff, Degree granting institution (dgg): Trent University
    Abstract: <p>Increased plant size is expected to have negative consequences for mating by increasing pollen transfer among the same plant. However, recent theoretical studies have demonstrated that this may not be true for clonal plants. Instead, clonal expansion could enhance outcrossing opportunities without increasing selfing by reducing distances to potential mates. I investigated how the spatial… more

    The Influence of nitrogen and sulfur on cadmium tolerance in Euglena gracilis: an RNA-Sequencing investigation

    Year: 2022, 2022
    Member of: Trent University Graduate Thesis Collection
    Name(s): Creator (cre): Kennedy , Victoria, Thesis advisor (ths): Saville, Barry, Degree committee member (dgc): Emery, Neil, Degree committee member (dgc): Donaldson, Michael, Degree committee member (dgc): Farrow, Scott, Degree granting institution (dgg): Trent University
    Abstract: <p>Heavy metal pollution threatens human and ecosystem health. E. gracilis was investigated for its potential use in bioremediation due to its tolerance for heavy metals and ability to sequester them from the environment. E. gracilis can remove metals by producing metal binding compounds enriched in sulfur and nitrogen. In this thesis, E. gracilis cultures that were pretreated with elevated… more

    SARS-CoV-2 Protein-based Detection Using Localized Surface Plasmon Resonance

    Year: 2022, 2022
    Member of: Trent University Graduate Thesis Collection
    Name(s): Creator (cre): Lewis, Tyra Marie, Thesis advisor (ths): Martic, Sanela, Degree committee member (dgc): Emery, Neil, Degree committee member (dgc): Saville, Barry, Degree granting institution (dgg): Trent University
    Abstract: <p>During the COVID-19 pandemic, nucleic acid and antibody-based testing methods were heavily relied upon, but can be costly, time-consuming and exhibit high false -negative and -positive rates. Thus, alternative strategies are needed. Viral antigens such as the SARS-CoV-2 spike (S) glycoprotein are critical in the function of the virus and useful as diagnostic biomarkers for viral… more

    Using ultra high-resolution mass spectrometry to characterize the biosorbent Euglena gracilis and its application to dysprosium biosorption

    Year: 2021, 2021
    Member of: Trent University Graduate Thesis Collection
    Name(s): Creator (cre): Lewis, Ainsely G., Thesis advisor (ths): Gueguen, Celine, Degree committee member (dgc): Emery, Neil, Degree committee member (dgc): Balch , Gordon, Degree granting institution (dgg): Trent University
    Abstract: <p>Euglena gracilis is an enigmatic and adaptable organism that has great bioremediationpotential and is best known for its metabolic flexibility. The research done in this
    dissertation addresses (1) how growth conditions impact cellular composition, and (2)
    how chemometric approaches (such as statistical design of experiments and artificial
    neural networks) are viable alternatives to the… more

    The Effects of Recycled Media on Culture Growth and Hormone Profiles in Heterotrophic Euglena gracilis

    Year: 2020, 2020
    Member of: Trent University Graduate Thesis Collection
    Name(s): Creator (cre): Kuhne, Alexandra Margaret, Thesis advisor (ths): Emery, Neil, Degree committee member (dgc): Balch, Gordon, Degree committee member (dgc): Gueguen, Celine, Degree granting institution (dgg): Trent University
    Abstract: <p>The rapid expansion of the worldwide population has caused an urgent need for the development of new, more environment-conscious, food sources. In this context, algae, such as Euglena, are of interest thanks to their capacity to naturally produce essential nutrients such as proteins and oils commonly found in animals and plant sources. While these processes are currently being… more

    cis-Cytokinins from the tRNA-degradation pathway impact the phenotype and metabolome of Arabidopsis thaliana: evidence from AtIPT2 and AtIPT9 null mutants

    Year: 2019, 2019
    Member of: Trent University Graduate Thesis Collection
    Name(s): Creator (cre): Butler, Cody Wade, Thesis advisor (ths): Emery, Neil, Degree committee member (dgc): Stock, Naomi, Degree committee member (dgc): Ellis, David, Degree granting institution (dgg): Trent University
    Abstract: <p>Cis-isomers of the cytokinin plant hormone family are thought to have low activity or impact on plant growth and development. Mutants with independent silencing of the pathway leading to cis-CK (cis-cytokinin) were investigated at the phenotype and metabolite levels. Phenotypic deviations were noted in trichome development, fresh weight, rosette diameter, number of non-rosette leaves,… more

    Cytokinins in nematodes: the potential role of cytokinins in soybean (Glycine max) resistance to soybean cyst nematode (Heterodera glycines)

    Year: 2019, 2019
    Member of: Trent University Graduate Thesis Collection
    Name(s): Creator (cre): Rahman, Tamzida, Thesis advisor (ths): Emery, Neil, Degree committee member (dgc): Saville, Barry, Degree committee member (dgc): Kisiala, Anna, Degree granting institution (dgg): Trent University
    Abstract: <p>To investigate cytokinins (CKs) in nematodes, CK profiles of a free-living Caenorhabditis elegans and a plant parasitic Heterodera glycines (soybean cyst nematode, SCN) were determined at the egg and larval stages. SCN had higher total CK level than C. elegans; however, CKs in SCN were mostly inactive precursors, whereas C. elegans had more bioactive forms. This is the first study to… more

    The Effect of Carbon Source and Phytohormones on the in vitro Growth of Euglena Gracilis

    Year: 2018, 2018
    Member of: Trent University Graduate Thesis Collection
    Name(s): Creator (cre): Trzaskalski, Natasha, Thesis advisor (ths): Emery, Neil, Degree committee member (dgc): Balch, Gordon, Degree committee member (dgc): Gueguen, Celine, Degree granting institution (dgg): Trent University
    Abstract: <p>Microalgae are a promising source of valuable compounds relevant to biofuels, biomaterials, nutraceuticals as well as animal and human nutriment. Unfortunately, low cell density and slow growth result in reduced economic feasibility. Heterotrophic cell culturing using an organic carbon source in lieu of light has proven to be an effective alternative to photobioreactors; however, further… more

    Cytokinin biosynthesis, signaling and translocation during the formation of tumors in the Ustilago maydis-Zea mays pathosystem

    Year: 2018, 2018
    Member of: Trent University Graduate Thesis Collection
    Name(s): Creator (cre): Alimi, Ibraheem Oladipupo, Thesis advisor (ths): Saville, Barry, Thesis advisor (ths): Emery, Neil, Degree committee member (dgc): Morrison, Erin, Degree granting institution (dgg): Trent University
    Abstract: <p>Cytokinins (CKs) are hormones that promote cell division. During the formation of tumors in the Ustilago maydis-Zea mays pathosystem, the levels of CKs are elevated. Although CK levels are increased, the origins of these CKs have not been determined and it is unclear as to whether they promote the formation of tumors. To determine this, we measured the CK levels, identified CK… more

    The Regulation of Cytokinins During Kernel Development in High and Low Yielding Oat and Barley Lines

    Year: 2017, 2017
    Member of: Trent University Graduate Thesis Collection
    Name(s): Creator (cre): Paleczny, Andrea Rose, Thesis advisor (ths): Emery, Neil, Degree committee member (dgc): Yee, Janet, Degree committee member (dgc): Saville, Barry, Degree committee member (dgc): Olechowski, Henry, Degree granting institution (dgg): Trent University
    Abstract: <p>Cytokinins (CKs) are a family of plant phytohormones responsible for many areas of plant growth and development. There are four free base types of CKs found in higher plants, trans-zeatin (tZ), N6-(∆2-isopentenyl)adenine (iP), cis-Zeatin (cZ) and dihydrozeatin (DZ). CK biosynthesis is regulated by adenosine phosphate-isopentenyltransferase (IPT), which is encoded by a multi-gene family… more

    Carbon Exchange along a Natural Gradient of Deciduous Shrub Coverage in the Low-Arctic

    Year: 2016, 2016
    Member of: Trent University Graduate Thesis Collection
    Name(s): Creator (cre): Ge, Le, Thesis advisor (ths): Lafleur, Peter M, Degree committee member (dgc): Watmough, Shaun A, Degree committee member (dgc): Emery, Neil, Degree granting institution (dgg): Trent University
    Abstract: <p>Arctic terrestrial ecosystems have experienced substantial structural and compositional changes in response to warming climate in recent decades, especially the expansion of shrub species in Arctic tundra. Climatic and vegetation changes could feedback to the global climate by changing the carbon balance of Arctic tundra. The objective of this thesis was to investigate the influence of… more

    Bioremoval of copper and nickel on living and non-living Eugelna gracilis

    Year: 2016, 2016
    Member of: Trent University Graduate Thesis Collection
    Name(s): Creator (cre): Winters, Cameron, Thesis advisor (ths): Gueguen, Celine, Degree committee member (dgc): Sager, Eric, Degree committee member (dgc): Emery, Neil, Degree granting institution (dgg): Trent University
    Abstract: <p>This study assesses the ability of a unicellular protist, Euglena gracilis, to remove Cu and Ni from solution in mono- and bi-metallic systems. Living Euglena cells and non-living Euglena biomass were examined for their capacity to sorb metal ions. Adsorption isotherms were used in batch systems to describe the kinetic and equilibrium characteristics of metal removal. In living systems… more

    Hormonal Algae: A Source of Functional Fatty Acids

    Year: 2016, 2016
    Member of: Trent University Graduate Thesis Collection
    Name(s): Creator (cre): Ramphal, Kira Elizabeth, Thesis advisor (ths): Emery, Neil, Thesis advisor (ths): Narine, Suresh, Degree committee member (dgc): Sager, Eric, Degree granting institution (dgg): Trent University
    Abstract: <p>Based on an endogenous hormone study, three cytokinin type phytohormones; benzyladenine (BA), trans-zeatin (tZ) and methylthiol trans-zeatin (MeSZ), as well as abscisic acid (ABA) were exogenously added at three concentrations (10-7, 10-6 and 10-5 M) to cultures of Chlorella vulgaris in an attempt to alter growth rate, total lipid and fatty acid yields and fatty acid profile. Growth… more

    The Involvement of Endogenous Plant Hormones in The Regulatory Network of Fatty Acid Biosynthesis in Soybean Seed

    Year: 2016, 2016
    Member of: Trent University Graduate Thesis Collection
    Name(s): Creator (cre): Nguyen, Thien Quoc, Thesis advisor (ths): Emery, Neil, Thesis advisor (ths): Narine, Suresh, Degree granting institution (dgg): Trent University
    Abstract: <p>The activities of phytohormones during the reproductive phase have been partially clarified in seed physiology while the biological role of plant hormones in oil accumulation during seed development has been investigated in part only. In this research, fatty acid (FA) contents and hormone profiles, including abscisic acid (ABA) and cytokinins (CKs) of seed samples in four different… more

    Purification and Identification of Selenium-containing C-phycocyanin from Spirulina: Implications for Bioaccumulation and Ecotoxicity

    Year: 2014, 2014
    Member of: Trent University Graduate Thesis Collection
    Name(s): Creator (cre): Farell, Jana, Thesis advisor (ths): Wallschläger, Dirk, Degree committee member (dgc): Rafferty, Steven, Degree committee member (dgc): Emery, Neil, Degree granting institution (dgg): Trent University
    Abstract: <p>Selenium is an essential trace nutrient to many organisms, yet in high concentrations it is toxic. Organic selenium is more bioavailable to aquatic biota than inorganic selenium, but is usually found in much lower concentrations. Algae are known to biotransform inorganic selenium into several organo-selenium compounds, but it is unknown whether any of these bioaccumulate in the food… more

    Factors Controlling Peat Chemistry and Vegetation Composition in Sudbury Peatlands after 30 Years of Emission Reductions

    Year: 2014, 2014
    Member of: Trent University Graduate Thesis Collection
    Name(s): Creator (cre): Barrett, Sophie Elizabeth, Thesis advisor (ths): Watmough, Shaun, Degree committee member (dgc): Sager, Eric, Degree committee member (dgc): Emery, Neil, Degree committee member (dgc): Hutchinson, Tom, Degree granting institution (dgg): Trent University
    Abstract: <p>Peatlands are prevalent in the Sudbury, Ontario region. Compared with the well documented devastation to the terrestrial and aquatic ecosystems in this region, relatively little work has been conducted on the peatlands. The objective of this research was to assess factors controlling peat and plant chemistry, and vegetation composition in 18 peatlands in Sudbury after over 30 years of… more