Trent University

Stormwater ponds (SWPs) are a common feature in new urban developments where they are designed to minimize runoff peaks from impervious surfaces and retain particulate matter. As a consequence, SWPs can be efficient at retaining particle-bound nutrients, but may be less efficient at retaining nutrients that are present primarily in the dissolved form, like nitrogen (N). However, the forms of nutrients (e.g. particulate vs. dissolved) likely differ with hydrologic and seasonal conditions and few studies have examined year-round differences in nutrient forms and concentrations at urban SWPs. In order to contrast total suspended solids (TSS), phosphorus (P) and nitrogen (N) levels between low and high flow conditions, sampling was conducted at an urban SWP in Peterborough, ON between November 2012 and October 2013. Only an increase in TSS levels at the outflow between low and high flow conditions was observed, as well as a decrease in TSS levels at the outflow compared to Inflow 1 under low flow conditions. Nitrate-N (NO3-N) was the dominant form of N entering the pond under all flow conditions, whereas the fraction of total-P (TP) that was particulate increased under high flow conditions. Nevertheless, the dissolved fraction of TP was consistently high in these urban inlets. Only NO3-N was significantly greater in the inflows than outflow and only under low flow conditions. Increases in the proportions of organic-N and ammonium-N

in the outlet suggest that biological processing is important for N retention.

Author Keywords: nitrogen, Ontario, phosphorus, stormwater ponds, total suspended solids

Ustilago maydis (DC) Corda is the causal agent of 'common smut of corn'. Completion of the U. maydis lifecycle is dependent on development inside its host, Zea mays. Symptoms of U. maydis infection include chlorosis and the formation of tumours on all aerial corn tissues. Within the tumours, thick-walled diploid teliospores form; these are the reproductive and dispersal agent for the fungus. U. maydis is the model to study basidiomycete biotrophic plant-pathogen interactions. It holds this status in part because of the completely sequenced 20.5 Mb genome; however, thorough genome annotation is required to fully realize the value of this resource. The research presented here improved U. maydis genome annotation through the analysis of cDNA library sequences and comparative genomics. These analyses identified and characterized pathogenesis-related genes, and identified putative meiosis genes. This enabled the use of U. maydis as a model for investigating 'host-induced' meiosis. Further, the cDNA library analyses identified non-coding RNAs (ncRNAs) and natural antisense transcripts (NATs). NATs are endogenous RNA molecules with regions complementary to a protein-coding transcript. Although NATs have been identified in a wide variety of mammals, plants, and fungi, very few have been functionally characterized. Over 200 U. maydis NATs were annotated by analyzing full-length cDNA sequences. NAT structural features were characterized. Strand-specific RT-PCR was used to detect NATs in U. maydis and in a related smut fungus, U. hordei. The data supported a common role for NATs in smut teliospore development, independent of the RNA interference pathway. Analysis of the expression of one U. maydis NAT, as-um02151, in haploid cells, led to a model for NAT function in U. maydis during teliospore dormancy. This model proposed NATs facilitate the maintenance of stored mRNAs through the formation of double-stranded RNA. In testing this model, it was determined that the deletion of two separate upstream regulatory regions, one of which contained a ncRNA (ncRNA1), altered NAT levels and decreased pathogenesis. These studies strengthened U. maydis as a model organism, and began the functional investigation of NATs in U. maydis, which identified a new class of fungal pathogenesis genes.

Author Keywords: cDNA library analysis, genome annotation, mRNA stability, natural antisense transcripts, pathogenesis, Ustilago maydis

This research investigates urban climate governance through a case study of climate change adaptation policy making in the Canadian municipality of Vancouver, British Columbia (BC). It investigates the context in which the City of Vancouver was motivated to develop its climate change adaptation strategy (CCAS) by exploring the motivating factors and drivers behind the formulation of this plan. The research approach involved content analysis of policy documents underlying the CCAS as well as interviews with key politicians and policy makers familiar with the strategy. I conceptualize the development of the CCAS using Kingdon's Multiple Streams Framework, leading me to conclude that the convergence of three streams, namely: 1) knowledge of local climate change impacts and their cost to city assets, 2) political leadership and 3) green policy coordination in the City of Vancouver, created an opportunity that was seized upon by policy champions to address adaptation.

Author Keywords: adapting cities to climate change, climate change adaptation policy, climate change adaptation policy formulation, local climate governance, municipal adaptation plan, urban climate governance

Recent declines in growth and condition of Great Lakes' lake whitefish (Coregonus clupeaformis) have been linked to ecosystem-wide changes stemming from the invasion of dreissenid mussels. To test the influence of invasive mussels on this commercially important coregonid species, we collected archived scale samples from ten Great Lake locations and analyzed long-term changes in growth rates, delta 13C and delta 15N stable isotope ratios before and after mussel establishment. There was a decrease in pre-maturation growth after establishment in all four locations where we examined back-calculated growths. In six of the seven locations with dreissenid populations, a significant increase in delta 13C and a significant decrease in delta 15N was found. In dreissenid-absent locations of Lake Superior, we did not see changes in growth or isotope ratios indicative of a major regime shift. Observed shifts in isotopic signatures provide evidence for an increased reliance on nearshore food sources and shallower depth distribution as a result of dreissenids, which likely contributed to lowered growth of lake whitefish.

Author Keywords: Diporeia, Dreissenids, food web, Great Lakes, invasive species, lake whitefish

This thesis examines what the term "transgender narrative" represents at this particular time and location. I do this by examining various methods of transgender storytelling through different forms of media production, including autobiography, film, novels, and online platforms such as Tumblr and YouTube. In chapter one, I look at the production of novels and the value system by which they are judged ("gender capital") in transgender publics and counterpublics. In chapter two, I examine the history of the autobiography, along with the medical history closely associated with transgender identity and bodily transformation. The third chapter examines notions of violence and memorial behind the deaths of transgender people and the ways in which certain political revolutions are formed within a counterpublic. I deconstruct varying notions of identity, authorship, and cultural production and critically examine what it means to be transgender and what it means to tell stories about transgender people. I will conclude with how these stories are being shaped through social media to become more innovative and move away from the rigid value system of gender capital previously mentioned.

Author Keywords: autobiography, gender, sex, social media, transgender, transsexual

In 1915 U.S. Marines invaded Haiti. Driven first by the epidemiological dangers in Haiti, health and medicine was made a central tenet in administering the occupation. Useful for protecting the American Marines from disease, the Service d'Hygiene (the occupation-era Public Health Service) also served a hegemonic purpose. By bringing American biomedicine to sick Haitians, the Service d'Hygiene built support for the occupation and helped foster long-term connections between Haiti and the United States. This hegemonic drive was made possible by the incorporation of non-state actors into the colonial project. To achieve this, the American authorities forged a development strategy for Haiti that was premised upon a relationship between the state and private institutions such as the Rockefeller Foundation. This strategy also encouraged Haiti to look to the United States for support, a goal successfully realized when Haitian politicians continued to do so even after the Marines left Haiti in 1934.

Author Keywords: Haiti, Hegemony, Imperialism, Public Health, Rockefeller Foundation, Service d'Hygiene

There is little research devoted to exploring psychology's historical and discursive development. Psychological knowledge is generally presented as the contributions of individuals, but without context. The social, political, and economic aspects of psychology's development are scarcely discussed, including how the discipline came to be considered a science. This thesis project explored the history of the development of psychology. Specifically, psychology's claim to scientificity via the appropriation of the medical model of disease, and accordingly, the instantiation of the categorical paradigm of mental illness were examined. The discontinuous events that shaped psychology and its hallmark of scientificity were explored, including extensive concept transformations, political agendas, and marketing strategies. These practices were then explored in a practical way using the conception of clinical depression and the role of antidepressants as the first-line treatment for depression in the USA. This exploration revealed psychology's socio-historical contingencies and its agenda of prediction and control.

Author Keywords: Categorical Paradigm, Concept Transformations, Historicity, Knowledge Products, Psychology, Scientificity

The fate of silver nanoparticles (AgNPs) in surface waters determines the ecological risk of this emerging contaminant. In this research, the fate of AgNPs in lake mesocosms was studied using both a continuous (i.e. drip) and one-time (i.e. plug) dosing regime. AgNPs were persistent in the tested lake environment as there was accumulation in the water column over time in drip mesocosms and slow dissipation from the water column (half life of 20 days) in plug mesocosms. In drip mesocosms, AgNPs were found to accumulate in the water column, periphtyon, and sediment according to loading rate; and, AgNP coating (PVP vs. CT) had no effect on agglomeration and dissolution based on filtration analysis. In plug mesocosms, cloud point extraction (CPE), single-particle-inductively coupled mass spectroscopy (spICP-MS), and asymmetrical flow field-flow fractionation (AF4-ICP-MS) confirmed the temporal dissolution of AgNPs into Ag+ over time; however, complexation is expected to reduce the toxicity of Ag+ in natural waters.

Author Keywords: AF4-ICP-MS, cloud point extraction, fate, mesocosms, silver nanoparticles, SP-ICP-MS

The Beat Generation shaped, and was shaped by, the post-WWII containment culture that arose in 1950s America. This so-called cultural containment reflected the social, political, and economic factors that were unique to the post-WWII period and are often

considered concurrent to post-war McCarthyism, which promoted a national ideology of exclusionism that was foremost opposed to the threat of Communism. I propose in my thesis that containment was a major influence in the rhetoric of resistance that is found

within the most prominent works of the Generation. My thesis also looks at the how Beat literature shifted from the counterculture to the mainstream and the impact that celebrity had on the Generation. When the Beats achieved literary fame their counterculture

represented the forefront of the New Left and was synonymous with succeeding protest cultures of the 1960s.

Author Keywords: Beat Generation, Cold War, Containment Culture, McCarthyism, Postmodernism, Second Wave Feminism

This study examines interaction and accommodation between Western Christians and Muslims in the Levant between the Second and Third Crusades, 1145 to 1192, examining three groups: short term crusaders, members of military orders, and permanent settlers. While members of these groups possessed several personal and group identities, most shared a prescriptive religious identity that encouraged a common goal: holy war for the protection of the Holy Land from Muslims, whom they identified as a distinct, enemy `other.' Despite these prescriptive beliefs, when Christians came into contact with Muslims, particularly following longer and more varied contact, most engaged in some convergent accommodation, such as diplomatic accommodation, development of shared languages and gestures, or admiration for chivalric qualities. Those settled in the Levant accepted the existing economic and social structures, assuming the roles of previous elites, adopting certain local customs, sharing sacred spaces, medical knowledge, or even developing personal ties with Muslims.

Author Keywords: Accommodation, Christianity, Crusades, Identity, Islam