Trent University

This thesis shows and emphasizes the importance of Indigenous Knowledge (IK) in informing collaborative efforts that promote sustainable economic development in Indigenous communities. It tells the story of a participative research study undertaken with six Tseltal communities located in the Region Selva of Chiapas, Mexico, in the context of the Covid19 pandemic of 2020 and early 2021. In this study, the research participants reflect on their endeavours pursuing projects focused on the economic self-sufficiency of their communities. Their initiatives, which are deeply grounded in Tseltal practices while accompanied by the local non-profit organization IXIM AC, focus on developing economically self-sustaining enterprises in self-organized groups led by local Indigenous women. The findings offer a deep immersion into two aspects that emerge from Tseltal knowledge: The Nucleus of Tseltal community wellbeing and the Four Elements of Buhts'an qu'inal (Tseltal community wellbeing). The study's results show that these two IK grounded aspects guide the participants' endeavours in developing sHachel jwohc' a'tel (Tseltal initiatives of entrepreneurship) while also enabling opportunities for gender transformative collaborative work and sustained engagement in local initiatives of sna'el ya'beyel stuc te bin ay ma'yuc (Tseltal economic development oriented to community wellbeing).

Author Keywords: Community Wellbeing, Indigenous Entrepreneurship, Indigenous Knowledges, Indigenous Women, Participative Action Research, Sustainable Development

Postclassic (AD 900-1500) Maya diet at Ka'kabish, Belize was examined using stable carbon and nitrogen isotope analysis of human bone collagen, and stable carbon isotope analysis of bone structural carbonate. Isotope data were compared to skeletal and dental indicators of diet and disease, and dietary differences among burials excavated from chultuns (B-2, C-1, C-2, and C-3) at Ka'kabish. Varying in dimensions, chultuns are characterized as multiple subterranean chambers carved into limestone bedrock, where re-entry was permitted through the removal of a capstone placed over a circular entrance. Due to poor preservation and commingling of human remains, diet and its relation to age, sex, and social status could not be explored. The general diet at Ka'kabish is consistent with the consumption of a diverse range of terrestrial plants and animals, in addition to marine resources. Relative to the other chultun burials, Chultun C-2 is an outlier, with a noticeably different diet, evidence for skeletal pathology, and absence of dental modifications. This study demonstrates a lack of significant dietary differences among Postclassic Maya sites in northern Belize, along with an apparent reliance on marine resources, further supporting the notion of a close association, and equal participation in a regional trading system with coastal sites that allowed for populations in this region to thrive during the Postclassic period.

Author Keywords: Ancient Maya, Bioarchaeology, Ka'kabish, Osteology, Postclassic, Stable Isotopes

Fatty acid analysis was performed on archaeological bone from various fauna from sites in the Canadian arctic to better understand the preservation of fatty acids and their potential applications to palaeoecological and palaeodietary studies. These data were complemented by analyses of modern bone and soft tissue samples from livestock and harp seals (Pagophilus groenlandicus). Results of modern analyses revealed that in terrestrial species, bone has inherently lower concentrations of most fatty acids relative to other soft tissues (adipose, marrow, and muscle). These analyses suggest that the distribution of fatty acids in bone is unique compared to other tissues, and the types and abundances of fatty acids in bone may be linked to dietary sources of lipids. Of the archaeological samples analyzed, terrestrial species (caribou [Rangifer tarandus]) generally exhibited higher concentrations of saturated fatty acids compared to marine species (ringed seals [Pusa hispida] and polar bears [Ursus maritimus]), whereas marine species had higher concentrations of monounsaturated fatty acids compared to terrestrial species. Results of analyses on both modern and archaeological samples provided insight into the degradation of fatty acids in bone, and the rapid loss of polyunsaturated fatty acids in particular. Because the abundances of fatty acids are likely altered in the burial environment, it is recommended that future analyses incorporate compound specific isotope analysis to focus on applications of fatty acids that are typically in the highest abundance and arguably have undergone the least amount of change, including palmitic (C16:0) and stearic acid (C18:0)

Author Keywords: Archaeological Science, Bone Lipids, Fatty Acid Analysis, GC-MS, Lipid Preservation, Palaeoecology

Evaluating the impact of climate change is arguably one of the main goals of conservation biology, which can be addressed in part by studying the demographic history of species in the region of interest. In North America, landscape and species composition during the most recent Pleistocene epoch was primarily influenced by glaciation cycles. Glacial advance and retreat caused species ranges to shift as well, leaving signatures of past population bottlenecks in the genetic code of most species. Genomic tools have shown to be important tools for understanding these demographic events to enhance conservation biology measures in several species. In my thesis I first reviewed the state of ungulate genomics, with a focus on how such data sets can be used in understand demography, adaptation, and inform conservation and management. Importantly, the review introduces key analyses like the pairwise sequentially Markovian coalescent and features like variation in antlers and horns and selection pressures that are used throughout subsequent chapters. Using the North American mountain goat as a model species, I then explored the genomic and phenotypic variation in this alpine specialist mammal. Starting with the generation of the first genome assembly for the mountain goat, I identified genes unique to the mountain goat and modeled demographic history going back millions of years using a pairwise sequentially Markovian coalescent approach. Species' effective population size generally paralleled climatic trends over the past one hundred thousand years and severely declined to under a thousand individuals during the last glacial maximum. Given the biological importance of horns in mountain goats and the recent scientific interest in genetic basis of headgear, I analyzed over 23,000 horn records from goats harvested in British Columbia, Alaska and Northwest Territories from 1980 to 2017. Overall, variation in horn size over space and time was low; goats harvested further North had shorter horn lengths and smaller horn circumferences in one year old and 4 years and older age classes and 4 years and older age class, respectively. Proximity of roads, which was used as an indicator of artificial selection, had a small effect on horn size, with larger horns being harvested closer to major roads. Finally, I used two range-wide genomic data sets sequenced with a whole genome re-sequencing and reduced representation approaches to provide estimates of genetic diversity, contemporary effective population sizes and population structure. These insights can help inform management and will potentially make an impact in preserving the mountain goat.

Author Keywords: genome assembly, horn size, Oreamnos americanus, population demography, reduced representation sequencing, whole genome resequencing

Recent agricultural land use change in east-central Ontario, including the expansion of intensive agriculture (corn and soybean crops) and tile drainage (TD) infrastructure, may alter the fluxes of both phosphorus (P) and nitrogen (N) to the Lower Great Lakes. Through intensive monitoring of several sub-watersheds that encompassed a range of row crop and TD extents, this study examined differences in stream N and P concentrations both seasonally and during variable flow conditions, to better understand land use/land cover (LULC) relationships with nutrient export. There was no clear relationship between total P (TP; concentration or export) and agricultural LULC, and instead, TP delivery was highly sensitive to flow events, and TP concentrations (especially particulate P; PP) were significantly higher during event flow compared with baseflow. In contrast, the dissolved fraction of P (total dissolved P; TDP) and dissolved nitrogen as nitrate (NO3-N) were not sensitive to flow, but were instead positively related to row crop agriculture, and associations between NO3-N concentrations/export and tile-drained row crop area were particularly strong (concentration r2 = 0.93; export r2 = 0.88). Regression relationships showed that with every 10% increase in row crop area in watersheds, NO3-N and TDP flow-weighted concentrations increased by 0.34 mg/L and 1.5 µg/L, respectively. As well, the same 10% increase in row crop agriculture translated to an increase of NO3-N export of as much as 130 kg/km2. Geospatial records of TD are incomplete in east-central Ontario, which presents challenges for evaluating the contribution of TD to nutrient export. Understanding the response of nutrients to changes in agriculture and agricultural practices is an integral part of watershed management as rapid changes in both urban and agricultural LULC continue to put pressure on water quality in the Lower Great Lakes.

Author Keywords: East-Central Ontario, Nutrient Export, Row crops, Streams, Tile Drainage, Water Quality

Physical activity and classroom design changes are beneficial means to reduce stress, and enhance well-being. Results across some studies however, are mixed. Shanker Self-Reg™ supports the use of physical equipment and design as a means of managing arousal and tension levels. Previous research lacks rich description of educators' understanding of equipment and design, Self-Reg, and how this understanding affects the way it is implemented. In the current study, educators' understanding of Self-Reg, how this understanding influenced educators' approach to the school environment, and if one workshop was enough to inspire individuals to adopt a Self-Reg approach were explored using thematic analysis. Participants included educators from schools with beginner and intermediate-level experience in Self-Reg. The analysis produced eight themes and 8 sub-themes. Participants' knowledge of Self-Reg influenced their approach to their environments. Although one workshop may have inspired interest in the framework, it was not enough to shift educators' current practice.

Author Keywords: Arousal, Classroom, Self-Reg, Self-regulation, Stress, Teachers

Selenium is an essential trace nutrient to many organisms, yet in high concentrations it is toxic. Organic selenium is more bioavailable to aquatic biota than inorganic selenium, but is usually found in much lower concentrations. Algae are known to biotransform inorganic selenium into several organo-selenium compounds, but it is unknown whether any of these bioaccumulate in the food chain. In this study, selenium was incorporated into the methionine residues of an algal photosynthetic protein, c-phycocyanin from Spirulina spp. The extent of selenium incorporation was quantified by inductively coupled plasma-mass spectrometry (ICP-MS), and the protein was identified using electrospray mass spectrometry (ES-MS).

C-phycocyanin was isolated and purified from Spirulina with a final recovery of 20-30 % of the total c-phycocyanin present. Selenomethionine replaced 92.8% ± 1.22 of the methionine residues in c-phycocyanin when grown in 2.5 ppm sodium selenite. ES-MS was used to obtain protein spectra, and pure c-phycocyanin was identified. Data of full scans provided estimated masses of both protein subunits--α-chain measured at 18,036 Da; β-chain measured at 19,250 Da--close to the theoretical masses. Protein fragmentation by collision-induced dissociation and electron capture dissociation provided approximately 52 % amino acid sequence match with c-phycocyanin from Spirulina platensis. This study demonstrates the incorporation of selenium into an algal protein, and the identification of c-phycocyanin using electrospray ionization-mass spectrometry.

This thesis seeks to understand the influences of environmental variables on site location selection during the Late Woodland period (ca. A.D. 1000-1650) in south-central Ontario, specifically variables considered to be favourable to maize agriculture. Four analyses were undertaken: a geographic information system (GIS) comparative analysis of Late Woodland sites compared to random points; population estimates of four sites for which settlement pattern data was available; maize consumption estimates for these same sites, and; a maize resources catchment analysis of these sites. The analysis conducted did not produce conclusive results to answer questions related to maize-driven site selection, however it did show that requirements for maize resources at these sites could have been met in catchment areas of a 500 m radius, in one case in 250m. The results led to an important question for future research: if agricultural needs were not driving settlement location selection in this area, what was?

Author Keywords: Environmental Modeling, GIS, Late Woodland, Maize Agriculture, Movement of Communities, Ontario Archaeology

Nitrogen is a major constituent of agricultural fertilizers, and nitrogen inputs to stream water via runoff and groundwater lead to a variety of negative environmental impacts. In order to quantify the movement of nitrogen through aquatic food webs, fourteen streams with varying land uses across South-Central Ontario were sampled for two species of fish, freshwater mussels, and water for measurement of isotope ratios of δ15N and δ13C. I found that nitrogen isotopes in fish, water, and mussels were related to the percentage of riparian monoculture, and that carbon isotopes were unrelated to monoculture. Though all species were enriched as monoculture increased, the rate of δ15N enrichment as monoculture increased did not vary between species. This study has improved our understanding of how monoculture affects nutrient enrichment in stream food webs, and assesses the validity of using nitrogen isotopes to measure trophic positions of aquatic organisms across an environmental gradient.

Author Keywords: agriculture, fish, food webs, nitrogen, stable isotopes, streams

My thesis examines anthropomorphism and many avenues in which humans represent nonhumans to evaluate their own lives. Using Isabella Rossellini's Green Porno webseries, a collection of two-minute films starring Rossellini as a multitude of nonhumans with costumes transforming her into nonhuman, I posit that a new form of anthropomorphism -- one that values the nonhuman in all his or her nonhumanity -- is emerging in contemporary media. Rossellini describes the mating, seduction, and maternal instincts of these nonhumans, regularly drawing parallels between nonhuman and human behavior and uncovering crucial intersections in femininity, masculinity, queer theory, and abjection. In more recent films, I see Rossellini performing certain nonhumans to critique particular characteristics of Western human society and incredulously addressing the human viewer as a member of a species that might not be as high in the caste system of living beings as he or she is led to believe. In turning this sense of grotesque Otherness onto the human, I identify Rossellini as engaging in counterabjection, or the reversal of extreme degradation often projected upon nonhuman bodies by humans.

Author Keywords: abjection, animal studies, nonhuman, queer studies