Year: 2024, 2024
Member of: Trent University Graduate Thesis Collection
Abstract: <p>Giardia intestinalis is a parasitic protozoan that possesses a flavohemoglobin (gFlHb), an enzyme that plays a role in the detoxification of reactive nitrogen species (RNS) and reactive oxygen species (ROS) via its nitric oxide dioxygenase (NOD) activity as well as its NADH-oxidase activity. This enzyme is a potential target for imidazole-based antigiardial drugs that act as ligands of… more Full Text: Rapid Assays to Test for Flavohemoglobin Inhibitors A thesis submitted to the Committee of Graduate Studies in partial fulfillment of the requirements for the degree of Master of Science in the Faculty of Arts and Science Trent University Peterborough, …
Year: 2021, 2021
Member of: Trent University Graduate Thesis Collection
Abstract: <p>Transactive response (TAR) DNA-binding protein of 43 kDa (TDP-43) pathology, including fibrillar aggregates and mutations, develops in amyotrophic lateral sclerosis (ALS) and is characterized by hyperphosphorylation and aggregation patterns, a mechanism largely understudied. In addition, ALS remains without a cure. Herein, in vitro aggregation of phosphorylated TDP-43 was explored, and… more Full Text: IMMUNOTHERAPIES TARGETING THE AMYOTROPHIC LATERAL SCLEROSIS-ASSOCIATED PROTEIN TDP-43 A Thesis Submitted to the Committee of Graduate Studies in Partial Fulfillment of the Requirements for the Degree of Master of Science in the Faculty of Arts and …
Year: 2021, 2021
Member of: Trent University Graduate Thesis Collection
Abstract: <p>Giardia intestinalis possesses four isotypes of cytochrome b5 (gCYTB-I-IV) that differ from their mammalian counterparts, suggesting different functions in this protozoan parasite. Although the recently discovered Giardia flavoenzyme, GiOR-1, reduces these cytochromes, its properties have not been thoroughly studied, owing to the difficulty in its expression. Here I describe successful… more Full Text: Expression of Giardia intestinalis flavoenzyme GiOR-1 and characterization of its electron transfer properties A thesis submitted to the Committee on Graduate Studies in partial fulfillment of the requirements for the degree of Master of Science in the …
Year: 2021, 2021
Member of: Trent University Graduate Thesis Collection
Abstract: <p>The onset of neurodegenerative diseases such as Alzheimer's disease (AD) and amyotrophic lateral sclerosis (ALS) are typically characterised by the aggregation of protein biomarkers into cytotoxic fibrils. Novel means of analysing these biomarkers are needed to expand the literature toward earlier diagnosis of these conditions. Electrochemical sensors could offer the sensitivity and… more Full Text: ELECTROCHEMICAL BIOSENSORS FOR NEURODEGENERATIVE DISEASE BIOMARKERS: INVESTIGATIONS INTO THE ORIGIN AND DIAGNOSIS OF ALZHEIMER’S DISEASE AND AMYOTROPHIC LATERAL SCLEROSIS A Thesis Submitted to the Committee on Graduate Studies in Partial Fulfillment of …
Year: 2019, 2019
Member of: Trent University Graduate Thesis Collection
Name(s): Creator (cre): Sajer, Bayan, Thesis advisor (ths): Yee, Janet, Degree committee member (dgc): Rafferty, Steven, Degree committee member (dgc): Bates, Holly, Degree committee member (dgc): Brunetti, Craig, Degree granting institution (dgg): Trent University Abstract: <p>The parasitic protist Giardia intestinalis has five heme proteins: a flavohemoglobin and several isotypes of cytochrome b5. While the flavohemoglobin has a role in counteracting nitric oxide, the functions of the cytochromes (gCYTb5s) are unknown. In this study, the protein level and cellular localization of three gCYTB5 isotypes (gCYTb5-I, II and III) and flavohemoglobin were examined… more Full Text: THE EFFECT OF NITROSATIVE STRESS ON HEME PROTEIN EXPRESSION AND LOCALIZATION IN GIARDIA INTESTINALIS A Thesis Submitted to the Committee on Graduate Studies in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy in the Faculty …
Year: 2019, 2019
Member of: Trent University Graduate Thesis Collection
Abstract: <p>Aquatic contaminant mobility and biological availability is strongly governed by the complexation of organic and inorganic ligands. Dissolved organic matter (DOM) is a complex, heterogeneous mixture of organic acids, amino acids, lipids, carbohydrates and polyphenols that vary in composition and can complex to dissolved metals thereby altering their fate in aquatic systems. The research… more Full Text: Molecular Composition of Dissolved Organic Matter Controls Metal Speciation and Microbial Uptake A dissertation submitted to the Committee of Graduate Studies in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the faculty …
Year: 2017, 2017
Member of: Trent University Graduate Thesis Collection
Name(s): Creator (cre): Dayer, Guillem Sébastien, Thesis advisor (ths): Yee, Janet, Thesis advisor (ths): Rafferty, Steven, Degree committee member (dgc): Brunetti, Craig, Degree committee member (dgc): Saville, Barry, Degree granting institution (dgg): Trent University Abstract: <p>Giardia intestinalis is an anaerobic protozoan that lacks common eukaryotic heme-dependent respiratory complexes and does not encode any proteins involved in heme biosynthesis. Nevertheless, the parasite encodes several hemeproteins, including three members of the Type II cytochrome b5 sub-group of electron transport proteins found in anaerobic protist and amitochondriate organisms.… more Full Text: INTERACTOME STUDY OF GIARDIA INTESTINALIS CYTOCHROMES B5 TRENT UNIVERSITY Peterborough, Ontario, Canada © Copyright by Guillem Dayer 2017 Environmental and Life Sciences Ph.D. Graduate Program May 2017 ABSTRACT Interactome study of Giardia intestinalis …
Year: 2017, 2017
Member of: Trent University Graduate Thesis Collection
Name(s): Creator (cre): Desantis, Lanna Marie, Thesis advisor (ths): Bowman, Jeff, Thesis advisor (ths): Burness, Gary, Degree committee member (dgc): Rafferty, Steven, Degree committee member (dgc): Wilson, Paul, Degree committee member (dgc): Boonstra, Rudy, Degree committee member (dgc): Vijayan, Mathilakath M, Degree granting institution (dgg): Trent University Abstract: <p>Across vertebrate taxa, the hypothalamic-pituitary-adrenal axis (or the stress axis) is highly conserved, and is central to vertebrate survival because it allows appropriate responses to psychological stressors. Habitat shapes successful physiological and ecological strategies, and to appreciate how individual species respond to stressors in their environment, it is essential to have a… more Full Text: STRESS AXIS FUNCTION AND REGULATION IN NEW WORLD FLYING SQUIRRELS: AN ASSESSMENT OF ACUTE STRESS RESPONSE, NEGATIVE FEEDBACK, AND THE ROLE OF CORTICOSTEROID-BINDING GLOBULIN A Thesis Submitted to the Committee on Graduate Studies in Partial Fulfillment of …
Year: 2017, 2017
Member of: Trent University Graduate Thesis Collection
Abstract: <p>The parasitic protist Giardia intestinalis lacks most heme proteins yet encodes a flavohemoglobin (gFlHb) that converts nitric oxide to nitrate and likely protects the cell from nitrosative stress. In this work an antibody raised against gFlHb was used to examine both changes in gFlHb expression levels and intracellular localization in Giardia in response to nitrosative stress. Giardia… more Full Text: FLAVOHEMOGLOBIN EXPRESSION IN GIARIDA INTESTINALIS EXPOSED TO NITROSATIVE STRESS A Thesis Submitted to the Committee of Graduate Studies in Partial Fulfillment of the Requirements for the Degree of Master of Science in the Faculty of Arts and Science …
Year: 2016, 2016
Member of: Trent University Graduate Thesis Collection
Abstract: <p>The epoxides of oligomeric self-metathesized soybean oil (MSBO) and cross-metathesized palm oil (PMTAG) and canola oil (CMTAG) containing terminal double bonds were used to produce nonisocyanate polyurethanes (NIPUs) as well as anhydride, amine and thiol-cured epoxies. </p><p>The synthesized NIPUs displayed metal adhesive properties for CMTAG and MSBO with MSBO being favoured… more Full Text: BIO-BASED POLYMERS FROM EPOXIDIZED VEGETABLE OILS MODIFIED BY METATHESIS A Thesis Submitted to the Committee on Graduate Studies in Partial Fulfillment of the Requirements for the Degree of Master of Science in the Faculty of Arts and Science TRENT …
Year: 2015, 2015
Member of: Trent University Graduate Thesis Collection
Abstract: <p>Ustilago maydis is a basidiomycete smut fungus and the causal agent of common smut of corn. Disease progression and fungal development in this pathogen occur in planta, terminating in the production of dormant teliospores. Dormant spores of many fungi are characterized by reduced metabolic activity, which is restored during spore germination. The transition out of dormancy requires the… more Full Text: NATURAL ANTISENSE TRANSCRIPTS TO NUCLEUS-ENCODED MITOCHONDRIAL GENES ARE LINKED TO Ustilago maydis TELIOSPORE DORMANCY A Thesis Submitted to the Committee on Graduate Studies in Partial Fulfillment of the Requirements for the Degree of Master of Science …
Year: 2014, 2014
Member of: Trent University Graduate Thesis Collection
Abstract: <p>Selenium is an essential trace nutrient to many organisms, yet in high concentrations it is toxic. Organic selenium is more bioavailable to aquatic biota than inorganic selenium, but is usually found in much lower concentrations. Algae are known to biotransform inorganic selenium into several organo-selenium compounds, but it is unknown whether any of these bioaccumulate in the food… more Full Text: Purification and Identification of Selenium-containing C-phycocyanin from Spirulina: Implications for Bioaccumulation and Ecotoxicity A Thesis Submitted to the Committee on Graduate Studies in Partial Fulfillment of the Requirements of the Degree of …
