Year: 2025, 2025
Member of: Trent University Graduate Thesis Collection
Abstract: <p>Frog virus 3 (FV3) belongs to the genus Ranavirus within the Iridoviridae family.Its 105,903 base genome encodes 98 open reading frames (ORFs), including ORF 97R, a
putative apoptosis regulator sharing 31% structural similarity with the anti-apoptotic Bcl-
2 family protein, myeloid cell leukemia 1 (Mcl-1). 97R contains a BH1 domain,
implicated in apoptosis regulation, and a predicted C-… more Year: 2025, 2025
Member of: Trent University Graduate Thesis Collection
Abstract: <p>The TATA-binding protein (TBP) is a key regulator of eukaryotic transcription initiation. The TBP homolog from Giardia intestinalis (gTBP) is highly divergent among all TBPs; notably lacking three of the four phenylalanine residues to unwind double- stranded DNA. I show that gTBP preferentially binds to single-stranded DNA (ssDNA) in two modes based on sequence and protein concentration… more Year: 2025, 2025
Member of: Trent University Graduate Thesis Collection
Abstract: <p>Crop losses due to pathogens, pests, and weeds account for 20–40% of global production, with fungal pathogens responsible for the most significant yield reductions and economic impact. The diseases caused by fungi spread through dormant spores, which protect its genetic material under adverse conditions. Dormancy is maintained until favorable germination conditions are met. Despite their… more Year: 2024, 2024
Member of: Trent University Graduate Thesis Collection
Abstract: <p>The neuronal ceroid lipofuscinoses (NCLs), commonly known as Batten disease, are a family of fatal neurodegenerative disorders that primarily affect children. Several subtypes of NCLs have been reported, each being caused by a mutation in a distinct ceroid lipofuscinosis neuronal (CLN) gene; this results in aberrant lysosome function and the accumulation of lipoprotein aggregates (known… more Full Text: Insights from Dictyostelium: Examining the role of cellular stress in Batten disease A Thesis Submitted to the Committee of Graduate Studies in Partial Fulfillment of the Requirements for Degree of Master of Science in the Faculty of Arts and Science …
Year: 2024, 2024
Member of: Trent University Graduate Thesis Collection
Abstract: <p>Giardia intestinalis is the causative agent of a diarrheal disease in mammals, but the mechanisms of disease pathogenesis are unclear. While proteins secreted by Giardia affect the host cells, the potential of hormone secretion has not been investigated to date. Cytokinins (CKs) are classified as phytohormones, but little is known about their role beyond plants. Mass spectrometry-based… more Full Text: The effect of cytokinins on the metabolite secretome of Giardia intestinalis during trophozoite growth, nutrient deprivation, and encystation A thesis submitted to the Committee of Graduate Studies in partial fulfillment of the requirements for the degree …
Year: 2024, 2024
Member of: Trent University Graduate Thesis Collection
Abstract: <p>The neuronal ceroid lipofuscinoses (NCLs), commonly known as Batten disease, are a family of fatal neurodegenerative disorders that primarily affect children. Several subtypes of NCLs have been reported, each being caused by a mutation in a distinct ceroid lipofuscinosis neuronal (CLN) gene; this results in aberrant lysosome function and the accumulation of lipoprotein aggregates (known… more Year: 2024, 2024
Member of: Trent University Graduate Thesis Collection
Abstract: <p>Giardia intestinalis is the causative agent of a diarrheal disease in mammals, but the mechanisms of disease pathogenesis are unclear. While proteins secreted by Giardia affect the host cells, the potential of hormone secretion has not been investigated to date. Cytokinins (CKs) are classified as phytohormones, but little is known about their role beyond plants. Mass spectrometry-based… more Year: 2022, 2022
Member of: Trent University Graduate Thesis Collection
Abstract: <p>Giardia intestinalis is a waterborne enteric parasite that lacks mitochondria and the capacity for heme biosynthesis. Despite this, Giardia encodes several heme proteins, including four cytochrome b5 isotypes (gCYTB5-I – IV) of unknown function. The aim of this thesis is to gain insight into the function of the Giardia cytochrome b5 isotype III (gCYTB5-III) that is found in the nucleus,… more Full Text: Interactome study of the Giardia intestinalis nuclear localized cytochrome b5 A Thesis Submitted to the Committee on Graduate Studies in Partial Fulfillment of the Requirements for the Degree of Master of Science in the Faculty of Arts and Science TRENT …
Year: 2022, 2022
Member of: Trent University Graduate Thesis Collection
Abstract: <p>Amino acids make up proteins, which are the building blocks of life. A balance of amino acids is needed to maintain a healthy state. Tyrosine (Tyr) is synthesized from the metabolism of phenylalanine, which is an essential amino acid, meaning it can only be obtained from the diet. It is related to many metabolic and neurodegenerative diseases. Tyr can undergo post-translational… more Full Text: USING FLUORESCENT CARBON DOTS FOR BIOSENSING APPLICATIONS OF AMINO ACIDS A Thesis Submitted to the Committee on Graduate Studies in Partial Fulfillment of the Requirements for the Degree of Master Science in the Faculty of Arts and Science TRENT …
Year: 2020, 2020
Member of: Trent University Graduate Thesis Collection
Abstract: <p>The waterborne protozoan Giardia intestinalis cycles between the environmentally-resistant and infectious cyst and the metabolically-active trophozoite that adheres to the epithelial lining of the small intestine. Adhesion can trigger the innate immune response in epithelial cells, including the synthesis of the free radical nitric oxide (NO) that inhibits cell proliferation and… more Full Text: Differential expression of cytochrome b5 proteins in Giardia intestinalis during nitrosative stress and encystation TRENT UNIVERSITY Peterborough, Ontario, Canada © Copyright by Kaitlyn Mowat 2020 Environmental and Life Sciences M. Sc. Graduate Program …
Year: 2019, 2019
Member of: Trent University Graduate Thesis Collection
Name(s): Creator (cre): Sajer, Bayan, Thesis advisor (ths): Yee, Janet, Degree committee member (dgc): Rafferty, Steven, Degree committee member (dgc): Bates, Holly, Degree committee member (dgc): Brunetti, Craig, Degree granting institution (dgg): Trent University Abstract: <p>The parasitic protist Giardia intestinalis has five heme proteins: a flavohemoglobin and several isotypes of cytochrome b5. While the flavohemoglobin has a role in counteracting nitric oxide, the functions of the cytochromes (gCYTb5s) are unknown. In this study, the protein level and cellular localization of three gCYTB5 isotypes (gCYTb5-I, II and III) and flavohemoglobin were examined… more Full Text: THE EFFECT OF NITROSATIVE STRESS ON HEME PROTEIN EXPRESSION AND LOCALIZATION IN GIARDIA INTESTINALIS A Thesis Submitted to the Committee on Graduate Studies in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy in the Faculty …
Year: 2018, 2018
Member of: Trent University Graduate Thesis Collection
Abstract: <p>Ustilago maydis is a biotrophic fungal plant pathogen that causes 'common smut of corn' disease. During infection, U. maydis develops a metabolic dependency on its host, relying on uptake of the carbon molecules provided within Zea mays tissues. The research presented indicated a requirement for metabolism of the pentose sugar D-xylose through functional investigation of a U.… more Full Text: FUNCTIONAL INVESTIGATION OF A Ustilago maydis XYLOSE METABOLISM GENE AND ITS ANTISENSE TRANSCRIPTS A thesis submitted to the Committee of Graduate Studies in partial fulfillment of the requirements for the degree of Master of Science in the faculty of …
Year: 2017, 2017
Member of: Trent University Graduate Thesis Collection
Name(s): Creator (cre): Dayer, Guillem Sébastien, Thesis advisor (ths): Yee, Janet, Thesis advisor (ths): Rafferty, Steven, Degree committee member (dgc): Brunetti, Craig, Degree committee member (dgc): Saville, Barry, Degree granting institution (dgg): Trent University Abstract: <p>Giardia intestinalis is an anaerobic protozoan that lacks common eukaryotic heme-dependent respiratory complexes and does not encode any proteins involved in heme biosynthesis. Nevertheless, the parasite encodes several hemeproteins, including three members of the Type II cytochrome b5 sub-group of electron transport proteins found in anaerobic protist and amitochondriate organisms.… more Full Text: INTERACTOME STUDY OF GIARDIA INTESTINALIS CYTOCHROMES B5 TRENT UNIVERSITY Peterborough, Ontario, Canada © Copyright by Guillem Dayer 2017 Environmental and Life Sciences Ph.D. Graduate Program May 2017 ABSTRACT Interactome study of Giardia intestinalis …
Year: 2017, 2017
Member of: Trent University Graduate Thesis Collection
Abstract: <p>To study the Giardia intestinalis cell cycle, counterflow centrifugal elutriation (CCE) was used to separate an asynchronous trophozoite culture into fractions enriched for cells at the different stages of the cell cycle. For my first objective, I characterized the appearance of a third peak (Peak iii) in our flow cytometry analysis of the CCE fractions that initially suggested the… more Full Text: STUDIES OF THE GIARDIA INTESTINALIS TROPHOZOITE CELL CYCLE A Thesis Submitted to the Committee of Graduate Studies in Partial Fulfillment of the Requirements for the Degree of Master of Science in the Faculty of Arts and Science TRENT UNIVERSITY …
Year: 2017, 2017
Member of: Trent University Graduate Thesis Collection
Abstract: <p>The amitochondrial protozoan, Giardia intestinalis, encodes four members of the cytochrome b5 (CYTB5) family of heme proteins of unknown function. While homology models can predict the likely fold of these proteins, supporting experimental evidence is lacking. The small size of the cytochromes (~16 kDa) makes them attractive targets for structural analysis by Electron Paramagnetic… more Full Text: NMR and EPR Studies on Cytochrome b5 Isotypes of Giardia intestinalis A thesis submitted to the Committee on Graduate Studies in Partial Fulfillment of the requirements for the degree of Master of Science in the Faculty of Arts and Science Trent …
Year: 2017, 2017
Member of: Trent University Graduate Thesis Collection
Abstract: <p>The parasitic protist Giardia intestinalis lacks most heme proteins yet encodes a flavohemoglobin (gFlHb) that converts nitric oxide to nitrate and likely protects the cell from nitrosative stress. In this work an antibody raised against gFlHb was used to examine both changes in gFlHb expression levels and intracellular localization in Giardia in response to nitrosative stress. Giardia… more Full Text: FLAVOHEMOGLOBIN EXPRESSION IN GIARIDA INTESTINALIS EXPOSED TO NITROSATIVE STRESS A Thesis Submitted to the Committee of Graduate Studies in Partial Fulfillment of the Requirements for the Degree of Master of Science in the Faculty of Arts and Science …
Year: 2017, 2017
Member of: Trent University Graduate Thesis Collection
Name(s): Creator (cre): Paleczny, Andrea Rose, Thesis advisor (ths): Emery, Neil, Degree committee member (dgc): Yee, Janet, Degree committee member (dgc): Saville, Barry, Degree committee member (dgc): Olechowski, Henry, Degree granting institution (dgg): Trent University Abstract: <p>Cytokinins (CKs) are a family of plant phytohormones responsible for many areas of plant growth and development. There are four free base types of CKs found in higher plants, trans-zeatin (tZ), N6-(∆2-isopentenyl)adenine (iP), cis-Zeatin (cZ) and dihydrozeatin (DZ). CK biosynthesis is regulated by adenosine phosphate-isopentenyltransferase (IPT), which is encoded by a multi-gene family… more Full Text: THE REGULATION OF CYTOKININS DURING KERNEL DEVELOPMENT IN HIGH AND LOW YIELDING OAT AND BARLEY LINES A Thesis Submitted to the Committee on Graduate Studies in Partial Fulfillment of the requirements for the Degree of Master of Science in the Faculty of …
Year: 2016, 2016
Member of: Trent University Graduate Thesis Collection
Abstract: <p>The aim of this work was to identify the DNA sequences recognized by the Giardia TBP (gTBP) in vivo by using a chromatin immunoprecipitation assay (ChIP). Since a specific antibody for the protein of interest is required for this assay, a company was contracted to produce and purify a custom polyclonal antibody from the immunization of rabbits. Recombinant GST-gTBP was produced at a… more Full Text: CHARACTERISATION OF THE GIARDIA TATABINDING PROTEIN - PREPARATION FOR AN IN VIVO APPROACH A Thesis Submitted to the Committee of Graduate Studies in Partial Fulfilment of the Requirements for the degree of Master of Science in the Faculty of Arts and …
Year: 2016, 2016
Member of: Trent University Graduate Thesis Collection
Abstract: <p>The flavohemoglobin in Giardia intestinalis (gFlHb) is the only known protozoan member of a protein class typically associated with detoxifying nitric oxide (by oxidation to nitrate) in bacteria and yeast. Mutants of the B10 tyrosine (Y30F) and E11 leucine (L58A), conserved residues thought to influence ligand binding, were expressed and studied using Resonance Raman (RR) spectroscopy.… more Full Text: Mutation of the B10 Tyrosine and E11 Leucine in Giardia intestinalis Flavohemoglobin A thesis submitted to the Committee of Graduate Studies in partial fulfillment of the requirements for the degree of Master of Science in the Faculty of Arts and Science …
