Year: 2019, 2019
Member of: Trent University Graduate Thesis Collection
Abstract: <p>Ustilago maydis (D.C.) Corda is a biotrophic pathogen that secretes effectors to establish and maintain a relationship with its host, Zea mays. In this pathosystem, the molecular function of effectors is well-studied, but the regulation of effector gene expression remains largely unknown. This study characterized Zfp1, a putative U. maydis Zn(II)2Cys6 transcription factor, as a modulator… more Year: 2018, 2018
Member of: Trent University Graduate Thesis Collection
Abstract: <p>Ustilago maydis is a biotrophic fungal plant pathogen that causes 'common smut of corn' disease. During infection, U. maydis develops a metabolic dependency on its host, relying on uptake of the carbon molecules provided within Zea mays tissues. The research presented indicated a requirement for metabolism of the pentose sugar D-xylose through functional investigation of a U.… more Year: 2017, 2017
Member of: Trent University Graduate Thesis Collection
Abstract: <p>Puccinia triticina, wheat leaf rust (WLR), is the most economically damaging fungal rust of wheat on a global scale. This study identified transcriptome changes in a recently emerged race of WLR in Ontario with a new virulence type relative to a possible ancestor race. Also, this study focused on detecting variation in candidate virulence genes and uncovering novel insight into WLR… more Year: 2016, 2016
Member of: Trent University Graduate Thesis Collection
Abstract: <p>The flavohemoglobin in Giardia intestinalis (gFlHb) is the only known protozoan member of a protein class typically associated with detoxifying nitric oxide (by oxidation to nitrate) in bacteria and yeast. Mutants of the B10 tyrosine (Y30F) and E11 leucine (L58A), conserved residues thought to influence ligand binding, were expressed and studied using Resonance Raman (RR) spectroscopy.… more Year: 2016, 2016
Member of: Trent University Graduate Thesis Collection
Abstract: <p>Giardia intestinalis is a protozoan parasite that causes waterborne diarrheal disease in animals and humans. It is an unusual eukaryote as it lacks the capacity for heme biosynthesis; nonetheless it encodes heme proteins, including three cytochrome b5 isotypes (gCYTB5s) of similar size. Homology modelling of their structures predicts increased heme pocket polarity compared to mammalian… more Year: 2016, 2016
Member of: Trent University Graduate Thesis Collection
Abstract: <p>The aim of this work was to identify the DNA sequences recognized by the Giardia TBP (gTBP) in vivo by using a chromatin immunoprecipitation assay (ChIP). Since a specific antibody for the protein of interest is required for this assay, a company was contracted to produce and purify a custom polyclonal antibody from the immunization of rabbits. Recombinant GST-gTBP was produced at a… more Year: 2016, 2016
Member of: Trent University Graduate Thesis Collection
Abstract: <p>Cytokinin biosynthesis in organisms aside from plant species has often been viewed as a byproduct of tRNA degradation. Recent evidence suggests that these tRNA degradation products may actually have a role in the development of these organisms, particularly fungi. This thesis examines the importance of cytokinins, a group of phytohormones involved in plant cell division and… more Year: 2015, 2015
Member of: Trent University Graduate Thesis Collection
Abstract: <p>Ustilago maydis is a basidiomycete smut fungus and the causal agent of common smut of corn. Disease progression and fungal development in this pathogen occur in planta, terminating in the production of dormant teliospores. Dormant spores of many fungi are characterized by reduced metabolic activity, which is restored during spore germination. The transition out of dormancy requires the… more Year: 2014, 2014
Member of: Trent University Graduate Thesis Collection
Abstract: <p>Interactions between hosts and pathogens play a crucial role in their adaptation, evolution and persistence. These interactions have been extensively studied in model organisms, yet it is unclear how well they represent mechanisms of disease response in primary vectors in natural settings. The objective of my thesis was to investigate host-pathogen interactions in natural host… more Year: 2014, 2014
Member of: Trent University Graduate Thesis Collection
Abstract: <p><italic>Ustilago maydis</italic> (DC) Corda is the causal agent of 'common smut of corn'. Completion of the <italic>U. maydis</italic> lifecycle is dependent on development inside its host, <italic>Zea mays</italic>. Symptoms of <italic>U. maydis</italic> infection include chlorosis and the formation of tumours on all aerial corn… more Year: 2014, 2014
Member of: Trent University Graduate Thesis Collection
Abstract: <p>Glycine max (soybean) is an economically important plant species that registers a relatively low yield/seed weight compared to other food and oil seed crops due to higher rates of flower and pod abortion. Alleviation of this abortion rate can be achieved by altering the sink strength of the reproductive organs of soybeans. Cytokinin (CK) plays a fundamental role in promoting growth of… more
