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Young children learn their emotion regulation skills by modeling and internalizing their caregivers' emotional competencies. Inadequate or problematic emotional competencies in parents can result in insufficient development of these competencies in children, which can

have severe consequences on multiple domains of their lives, including physical wellbeing. This study examined the relationship between emotional competencies, health care usage rates, somatization and health anxiety in the context of a family with young children. Participants were mothers of children 4-11 years old recruited in the community. The results revealed a relationship between mother's emotional competencies and mother and child's health care usage rates. Mother's health care usage rates were also linked to mother's health anxiety and child's somatic symptoms. These findings add to our understanding of the relationship between emotional competencies of parents and children, and the effects it can have on both mother's and child's physical wellbeing. Implications and avenues for future research are discussed.

Author Keywords: emotional competencies, health care usage, mother and child, somatization

Road mortality is one of the leading causes of global population declines in reptiles and amphibians. Stemming losses from reptile and amphibian road mortality is a conservation priority and mitigation is a key recovery measure. I developed a model of road mortalities relative to non-­‐mortalities, based on predictors varying across space (road surface type, traffic volume, speed limit, distance to wetland) and time (weather conditions, traffic volume). Herpetofauna road mortalities were recorded during daily bicycle and vehicle surveys to investigate the impact of roads on reptiles and amphibians within the Bruce Peninsula, Ontario in 2012 and 2013. A total of 2541 observations of herpetofauna on roads were recorded, 79% of which were dead. The major factor influencing turtle road mortality was proximity to the nearest wetland and dates early in the season (spring). For the Massasauga, high daily temperatures and low daily precipitation were associated with road mortality. The major factors driving colubrid snake mortality were also high daily temperature, low daily precipitation, as well as low speeds and paved roads. Frog and toad mortality was driven by proximity to wetland and late summer dates. These models will increase our understanding of factors affecting road losses of herpetofauna and serve as a basis for planned, experimental mitigation within the Bruce Peninsula.

Author Keywords: amphibians, hotspot, mitigation, reptiles, road ecology, road mortality

Trinucleotide repeats (TNRs) are a class of highly polymorphic microsatellites which occur in neutral and non-neutral loci and may provide utility for individual- and population-identification. Exonic trinucleotide motifs, in particular, offer additional advantages for non-human species that typically utilize dinucleotide microsatellite loci. Specifically, the reduction of technical artifacts, greater separation of alleles and greater specificity of amplification products leading to more efficient multiplexing and cross-taxa utilization. This study aims to identify and characterize polymorphic trinucleotide repeats and conserved primer sequences which are conserved across Cervidae (deer) species and their potential for individual identification in forensic wildlife investigations. Chapter one provides a broad introduction to trinucleotide microsatellites, chapter two deals with data-mining TNRs and chapter three applies the identified TNRs as genetic markers for individual identification. Results demonstrate proof-of-concept that exonic TNRs are capable of giving random match probabilities low enough to be employed in individual identification of evidentiary samples.

Author Keywords: DNA typing, Exons, Genetic Markers, Individual Identification, Trinucleotide, Wildlife Forensics

Bloodstain pattern analysis is fundamental to crime scene investigations by providing\ninsight into the estimation of the area of origin of a blood source. The selection of bloodstains from an impact pattern is the foundation from which the estimation of the area of origin is derived. The purpose of this research was to develop a theoretical model for stain selection and establish a more robust estimation for the area of origin in the analysis of impact patterns. This model was developed by using quantitative data from marked zones on the impact pattern target surface. The minimum distance between the known blood source location and a single bloodstain line of trajectory was calculated for every bloodstain within each pattern investigated. It has been determined that the zone location of a bloodstain is relevant for improving accuracy, and that stains with all alpha angles can be used to calculate an area of origin estimation. Finally, research within this thesis also validated directional analysis and investigated the number of stains that may be required for a precise area of origin estimation.

Author Keywords: Forensic science, Bloodstain, Impact pattern, Bloodstain pattern analysis, Statistics, Forensic Physics

Agricultural land application of biosolids recycles nutrients and organic matter to the soil, however the effect of treatment process on nutrient availability requires further research for better nutrient management. This study examined the bioavailability of nitrogen (N) and phosphorus (P) in alkaline treated biosolids (TB) when amended into three different soils. Despite a 45% reduction in total N and P content during treatment, TB did not show reduced N or P availability compared with sewage sludge (SS). Results of a corn growth experiment and a soil incubation showed that TB amendment resulted in little mineralization and generally net immobilization of N, and 2% total P availability to corn from TB. Results suggest that TB are not a source of bioavailable N in the short-term, but can be used as a P amendment for corn. Nutrient management of agricultural land receiving these materials should focus on P added and liming properties.

Author Keywords: Alkaline treated biosolids, Nitrogen, Phosphorus, Soil fertility

Microalgae are a promising source of valuable compounds relevant to biofuels, biomaterials, nutraceuticals as well as animal and human nutriment. Unfortunately, low cell density and slow growth result in reduced economic feasibility. Heterotrophic cell culturing using an organic carbon source in lieu of light has proven to be an effective alternative to photobioreactors; however, further improvement may be possible with the addition of growth promoting phytohormones. In this thesis, growth and endogenous hormone profiles in heterotrophic cultures of Euglena gracilis were evaluated using glucose and ethanol as carbon sources. Cytokinin (CK) and abscisic acid (ABA) were quantified by HPLC-ESI-MS/MS and compared to culture growth dynamics. Exogenous phytohormones treatments were also conducted to determine if they may mitigate nutrient reduction and improve growth. Phytohormones CK and ABA were purified and analyzed at seven points along the growth curve in small scale (250 mL flasks, 100 mL working volume) cultures. Among the key findings was that ethanol cultures undergoing exponential growth, primarily synthesize freebase cytokinins (FBCKs) and methylthiol-cytokinins (MeSCKs), while not producing detectable levels of ABA. In exogenous studies, dry biomass was positively influenced with the addition of exogenous ABA; however, the most notable result revealed the ability of transZ to alleviate nutrient reduction. These findings suggest a communication network in algal culture using FBCKs and MeSCKs, as well as the potential for exogenous hormone supplementation to increase growth rates and overall biomass productivity.

Author Keywords: abscisic acid, cytokinin, Euglena gracilis, heterotrophy, phytohormones

In 2012, the Rio Tinto aluminum smelter in Kitimat, British Columbia increased sulphur dioxide (SO2) emissions from 27 to 42 tonnes/day. An initial study was conducted to investigate the effect of the increased sulphur (S) deposition on forest soils. A key uncertainty of the initial study was mineral surface area estimations that were applied to critical load calculations. The current study investigates the effect of organic matter (OM) removal techniques on mineral surface area and the ability to predict mineral surface area using pedotransfer functions (PTFs). Mineral surface area was measured on bulk soil samples using BET gas-adsorption. Organic matter was removed from soil samples prior to surface area measurements using a sodium hypochlorite treatment (NaOCl), loss on ignition (LOI) and no treatment. Removal techniques were found to affect surface area measurements; decreasing in the order of LOI> untreated> NaOCl. Particle-size based PTFs developed from other regions were not significantly correlated with measured surface area. A regionally-specific particle-size based function had stronger predictive value of surface area measurements (adjusted R2=0.82). The PTF that best reflected surface area measurements of bulk soil for the Kitimat area used particle-size data as well as kaolinite, the most abundant clay mineral in the region. Surface area values estimated using the particle-size PTF were applied to the PROFILE model to calculate weathering rates. Weathering rates were then input to critical load calculations using steady-state mass balance. These estimates predicted that none of the 24 measured sites are receiving SO2 deposition in exceedance of their critical load.

Author Keywords: acid deposition, critical loads, mineral surface area, mineral weathering, pedotransfer functions, PROFILE

Since 1984, poetry slams have emerged as a politicized expressive movement of performing the personal and political through poetry competitions. Slams are also discursively spatialized, often represented as "spaces" that are "safe," "inclusive," etc. In this thesis, I investigate how, why, and to what effect the Peterborough Poetry Slam produces, consolidates, and challenges such "resistant spaces." Drawing on interviews and participant observation, I consider how the slam's reiterative practices facilitate its space-making by encouraging performances that resist, reimagine, and sometimes inadvertently reify dominant societal norms. I argue that this space-making is imperfect yet productive: though not resistant space in any straightforward or static way, the slam continuously produces possibilities to challenge norms and confront power. This thesis contributes to scholarship on performative space and creative resistance movements. In an era when political resistance to power structures is often silenced, this research offers insights of potential significance to other resistant space-makings.

Author Keywords: Nogojiwanong Peterborough, performative space, poetry slam, resistance, space-making, spoken word

The end of the Cold War and the global triumph of neoliberalism were accompanied by the evolution of certain themes in dystopian fiction. According to some of its advocates, such as Francis Fukuyama, neoliberalism's success signified the "end of history," understood as ideological evolution, since the decline of communism left Western liberal democracies without any major opposition in terms of global governing and discursive practices. This thesis critically compares neoliberal rhetoric concerning invisible power, the end of history, technology, freedom of consumption and the commodification of human relationships with the ideologies represented in four neoliberal dystopian works of fiction, namely Black Mirror, Feed, The Circle, and The Fat Years. These examples create a "one-dimensional" dystopian subject who is rendered incapable of possessing the utopian imagination necessary to organize political resistance, precisely as a result of the governance and discourse of neoliberalism.

Author Keywords: dystopia, dystopian fiction, dystopian subjectivity, neoliberalism, post cold war fiction, subjectivity

Phytohormones, Cytokinin (CK) and Abscisic acid (ABA), are best known for controlling plant growth and stress responses; but they also mediate various developmental perspectives in alga. Yet, their mode of action in algal adaptive strategies to heavy metal responses, their involvement in orchestration of phytohormone crosstalk remain largely unknown and a molecular framework of phytohormone-controlled heavy metal uptake is absent. I found that three metals known globally to contaminate aquatic ecosystems, nickel (Ni), lead (Pb), and cadmium (Cd), cause changes in the levels of endogenous CKs, ABA, auxins, and gibberellins (GAs) in the green alga Euglena gracilis. Exogenous ABA or CK (trans-zeatin) alleviated metal toxicity through improved metal uptake efficiency and the regulation of the endogenous CKs activity profiles and GAs activity. This new evidence suggests that E. gracilis possesses functional phytohormone signals and metabolic pathways that are under metal stress response. Exogenously applied ABA or CK provoked the coordinated activation of metal uptake, likely via enhanced accumulation of metal binding compounds (i.e., proline, glycine, cysteine containing peptides), which are effective for metal sequestration. Using untargeted metabolomics analysis and functional annotation, this thesis further established that, CK and ABA modified pathways and metabolites, which were mainly involved in metal acclimation and resistance. These modified metabolites that were under the influence of phytohormones in algal cells growing under metal stress conditions were associated with: lipid pathways, riboflavin metabolism, biosynthesis of cofactors/vitamin, and carbohydrate metabolism. Bioactive secondary compounds (e.g., terpenoids, alkaloids, flavonoids, carotenoids) were also modified in algal cells treated with phytohormones. The present study highlights that ABA and CKs are important regulators of algal metal accumulation/acclimation strategies based on increased metal uptake, enhanced CK metabolism, regulation of hormonal crosstalk and regulation of some core cellular metabolism pathways, all of which improve metal uptake efficiency. Finally, our results suggest that ABA and CK form a novel strategy for metal bioremediation techniques and for sourcing microalgal value-added metabolites.

Author Keywords: abscisic acid, cadmium, cytokinin, Euglena gracilis, lead, nickel