Trent University

Community-based research (CBR) is a method of discovery that can provide pragmatic methods of advocating for and enabling community change. CBR literature and practice has focused on securing educational and job skills training outcomes for students rather than the communities, and community outcomes CBR and partnership frameworks were truly meant to serve. This research evaluates the effectiveness of a research brokering organization, and the community outcomes that can be meaningfully related back to established partnerships and research. A linked contribution and realist evaluation were employed to consider the contributions of U-Links Centre for Community-Based Research to capacity building in Haliburton County, for host organizations, local municipalities and the public. A community survey (n=65), interviews with past project hosts and management committee members (n=26) anecdotal project exploration, internal document review, and participant observation from living in the region and working within the organization, offers qualitative and quantitative data to support this contribution narrative, while also theorizing key factors for developing projects with high contribution potential. Five key factors were found which can act as both contexts and mechanisms of community-based research mobilization: relevance, relationships, resources, rigour and reach.

Author Keywords: capacity building, community, community-based research, contribution analysis, evaluations, research impact

Blocking fossil fuel infrastructure projects like pipelines is increasingly being seen as a legitimate way for civil society groups to reduce global carbon emissions. This research project is an exploratory case study of the Trans Mountain pipeline in British Columbia and its opposition. My research question asks, 'What has each tactic/strategy of opposition in the campaign to stop the Trans Mountain pipeline expansion accomplished, and how have they been effective? How can they be done more effectively?' Through interviews and an autoethnography, my research explores the effectiveness of activists in this campaign. I analyze the results of my findings within social movement theory and other activist definitions of effectiveness from my literature review. The more significant findings from my research are that activists need to do a better job educating the public on the issue, need to direct more of their resources towards promoting a solution to the issue and make alliances with other movements and groups. This research project contributes to the literature on the effectiveness of oppositional strategies and tactics of pipeline resistance, as well as social movement theory.

Author Keywords: British Columbia, pipeline, protest, strategy, tactic, Trans Mountain

Invasive Phragmites australis ssp. australis (herein "Phragmites") has established and rapidly spread throughout many coastal areas of the Great Lakes. Known to displace native vegetation communities as it forms large, monotypic stands, Phragmites has a bad reputation when it comes to losses of biodiversity and habitat provision for wildlife. However, the extent to which Phragmites provides habitat for fish and invertebrates in coastal freshwater wetlands remains relatively unquantified. Thus, this study assessed whether fish assemblages and invertebrate communities in stands of Phragmites differ from those in stands of two native emergent vegetation communities, Typha spp. and Schoenoplectus spp. The findings showed significant differences in habitat variables among the vegetation communities in terms of water depth, macrophyte species richness, stem density and water quality. While abundance of the functional feeding group filterer-collectors was found to be significantly less in stands of Phragmites when compared to Schoenoplectus, no difference was observed in invertebrate taxa richness among vegetation communities. Lastly, no difference in fish assemblage or invertebrate community was detected when using multivariate analyses, implying that invasive Phragmites provides habitat that appears to be as valuable for fish and invertebrates as other emergent vegetation types in the St. Clair River Delta. The findings of this study will ultimately benefit the literature on invasive Phragmites and its role as fish habitat in freshwater wetlands, and aid management agencies in decisions regarding control of the invasive species.

Author Keywords: aquatic invasive species, aquatic macroinvertebrates, freshwater fish, freshwater wetlands, nMDS, Phragmites

Syrphids, also known as hover flies (Diptera: Syrphidae) are a diverse and widespread family of flies. Here, I report on their distributions from a previously understudied region, the far north of Ontario, as well as Akimiski Island, Nunavut. I used samples collected through a variety of projects to update known range and provincial records for over a hundred species, bringing into clearer focus the distribution of syrphids throughout this region. I also analysed a previously un-tested trap type for collecting syrphids (Nzi trap), and report on results of DNA analysis for a handful of individuals, which yielded a potential new species.

Author Keywords: Diptera, Ontario, range extension, Syrphidae

Competition is known to impact population dynamics through both indirect and direct interactions, and direct interactions can often lead to injury in one or both parties. As such, response to injury through tissue regeneration can be important for surviving post-competitive interaction. However, the impacts of outside factors like temperature and genome size (e.g. polyploidy) are not well studied, especially in syntopic systems. We addressed this knowledge gap by comparing regeneration rates of diploid Ambystoma laterale and triploid unisexual Ambystoma at two ecologically-relevant temperatures. Environmental factors appeared to have stronger effects on regeneration than ploidy level, but overall mass was impacted more strongly by ploidy level. Interestingly, there was an interaction between temperature and time within unisexuals that was absent when comparing different ploidy levels, implying temperature has a more complex effect on polyploids. This study supports the hypothesis that polyploid organisms are better equipped to respond to shifts in their environments, which can give them a competitive advantage at the northern range limit of this species complex.

Author Keywords: Ambystoma, Genome dosage, Hybrid vigor, Polyploidy, Thermal optimum, Tissue regeneration

Public transportation systems play important role in the quality of life of citizens

in any metropolitan city. However, public transportation authorities face

criticisms from commuters due to irregularities in bus arrival times. For example,

transit bus users often complain when they miss the bus because it arrived too

early or too late at the bus stop. Due to these irregularities, commuters may miss

important appointments, wait for too long at the bus stop, or arrive late for work.

This thesis seeks to predict the occurrence of irregularities in bus arrival times by

developing machine learning models that use GPS locations of transit buses provided

by the Toronto Transit Commission (TTC) and hourly weather data. We

found that in nearly 37% of the time, buses either arrive early or late by more than

5 minutes, suggesting room for improvement in the current strategies employed by

transit authorities. We compared the performance of three machine learning models,

for which our Long Short-Term Memory (LSTM) [13] model outperformed all

other models in terms of accuracy. The error rate for LSTM model was the lowest

among Artificial Neural Network (ANN) and support vector regression (SVR). The

improved accuracy achieved by LSTM is due to its ability to adjust and update the

weights of neurons while maintaining long-term dependencies when encountering

new stream of data.

Author Keywords: ANN, LSTM, Machine Learning

Predation risk can elicit a range of responses in prey, but to date little is known about breadth of potential responses that may arise under realistic field conditions and how such responses are linked, leaving a fragmented picture of risk-related consequences on individuals. We increased predation risk in free-ranging snowshoe hares (Lepus americanus) during two consecutive summers by simulating natural chases using a model predator (i.e., domestic dog), and monitored hare stress physiology, energy expenditure, behaviour, condition, and habitat use. We show that higher levels of risk elicited marked changes in physiological stress metrics including sustained high levels of free plasma cortisol which had cascading effects on glucose, and immunology, but not condition. Risk-augmented hares also had lowered daily energy expenditure, spent more time foraging, and decreased rest, vigilance, and travel. It is possible that these alterations allowed risk-exposed hares to increase their condition at the same rate as controls. Additionally, risk-augmented hares selected, had high fidelity to, and were more mobile in structurally dense habitat (i.e., shrubs) which provided them additional cover from predators. They also used more open habitat (i.e., conifer) differently based on locale within the home range, using familiar conifer areas within cores for rest while moving through unfamiliar conifer areas in the periphery. Overall, these findings show that prey can have a multi-faceted, highly plastic response in the face of risk and can mitigate the effects of their stress physiology given the right environmental conditions.

Author Keywords: behaviour, condition, daily energy expenditure, predator-prey interactions, snowshoe hare, stress physiology

While preferences for symmetry are seemingly universal, they can be seen at their most extreme among individuals high in trait incompleteness. As yet, it is unclear why incompleteness yields heightened symmetry preferences. Summerfeldt et al. (2015) speculated that individuals high in incompleteness may develop heightened preferences for symmetry due to its greater perceptual fluency. Accordingly, the aim of the present set of three experiments was to examine this relationship. Implicit preferences for symmetry were measured using a modified version of the Implicit Association Test (IAT) reported by Makin et al. (2012). Experiments 1 (N = 24) and 2 (N = 24) examined whether the general implicit preferences for symmetry and influence of perceptual fluency reported by Makin et al. (2012) extended to a within-subjects design. Experiment 3 (N = 86) examined whether trait incompleteness is related to greater implicit preferences for symmetric stimuli, and whether perceptual fluency affects this association. Results showed that incompleteness and implicit preferences were related, and that incompleteness-related differences in preferences were eliminated when the patterns were equally perceptually fluent, supporting the idea that incompleteness-related preferences for symmetry are linked to perceptual fluency. Implications of these findings are discussed.

Understanding the complex genomic architecture underlying quantitative traits can provide valuable insight for the conservation and management of wildlife. Despite improvements in sequencing technologies, few empirical studies have identified quantitative trait loci (QTL) via whole genome sequencing in free-ranging mammal populations outside a few well-studied systems. This thesis uses high-depth whole genome pooled re-sequencing to characterize the molecular basis of the natural variation observed in two sexually selected, heritable traits in white-tailed deer (Odocoileus virginianus, WTD). Specifically, sampled individuals representing the phenotypic extremes from an island population of WTD for antler and body size traits. Our results showed a largely homogenous genome between extreme phenotypes for each trait, with many highly differentiated regions throughout the genome, indicative of a quantitative model for polygenic traits. We identified and validated several potential QTL of putatively small-to-moderate effect for each trait, and discuss the potential for real-world application to conservation and management.

Author Keywords: evolution, extreme phenotypes, genetics, genomics, quantitative traits, sexual selection

Soils play a crucial role in ecosystem functioning, for example, soil minerals provide important provisioning and regulate ecosystem services. This study used major soil oxides from the North American Soil Geochemical Landscapes Project (n=560) to assess elemental associations and infer soil minerals through exploratory data analysis and to determined quantitative soil mineralogy using a normative method, Analysis to Mineralogy (n=1170). Results showed elemental variability of oxides across the provinces of Canada and strong correlations occurred between elements indicative of soil mineral composition (e.g., Silicon and Aluminium). Principal component analysis inferred soil minerals from soil oxides trends on biplots and classified minerals, generally, as carbonates, silicates, and weathered secondary oxides. Spatial variability in minerals (quartz, plagioclase, potassium feldspar, chlorite, and muscovite) was related to the underlying bedrock geology. The use of Analysis to Mineralogy led to a reliable method of quantifying soil minerals at a large scale.

Author Keywords: Analysis to Mineralogy, Exploratory data analysis, Normative procedures, North American Soil Geochemical Landscapes Project, Soil geochemistry, Soil mineralogy