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Accuracy and reliability of microscopic characteristics to identify Typha species and their hybrids
Advanced generation/backcrossed (non-F1) hybrids can be challenging to identify when their traits are similar to those of parental taxa, F1 hybrids, or both. This is particularly evident in the North American hybrid zone involving Typha latifolia, T. angustifolia, F1 T. × glauca and non-F1 hybrids. Cattails are challenging to differentiate based on gross morphological characteristics. Microscopic characteristics in female inflorescences have not been previously studied to differentiate parental taxa from non-F1 hybrids. To investigate whether researchers can use microscopic floret and bracteole characteristics for taxonomic identification, I compared pistillate flower length, bracteole length and width, and bracteole colour among taxa. I found that floret and bracteole characteristics can be useful for identifying T. latifolia but cannot accurately differentiate T. angustifolia and F1 T. × glauca from non-F1 hybrids. Further, a flowering bias can lead to the underestimation of the frequency of T. latifolia when using floral characters to examine the relative abundance of cattail taxa.
Author Keywords: advanced-generation hybrids, backcrossed hybrids, invasive species, morphology, species identification, Typha spp.
Cities of the Future or a Relic of the Past? The Universality of Low-Density Urbanism Among the Ancient Maya
Low-density urbanism is ubiquitous in the industrialized world, with suburbs and sprawling urban zones like the American Northeastern Seaboard being classified as such. Due to outsized environmental impacts and perceived unsustainability, this settlement pattern is often maligned. As one of the few prominent examples of agrarian-based low-density urbanism, the ancient Maya can provide a much-needed case study on the sustainability of low-density urbanism. Therefore, a thorough assessment of the universality of low-density urbanism among the ancient Maya is warranted. Maps of 11 Maya sites were collected from published sources, digitized, and used to calculate household group densities. No significant difference was observed between Classic and Postclassic sites, but sites in the northern Lowlands were significantly denser than those in the southern Lowlands. Additionally, no significant inverse correlation was found between site density and area, which would be expected if low-density urbanism was universal among the ancient Maya.
Author Keywords: Household Archaeology, Low-Density Urbanism, Maya, Settlement Archaeology
Concentration-Dependent Effects of Cadmium on Mouse Angiogenesis In Vitro
Cadmium is a toxic metal that has detrimental effects on blood vessel development and function. To examine the effect of varying concentrations of cadmium on angiogenesis, two in vitro assays were used. First, developing yolk sac blood vessels were studied in gestation day 8 mouse embryos exposed to medium alone, 1.25, or 1.75 μM cadmium chloride (CdCl2). Embryos exposed to 1.25 μM cadmium experienced a significant increase in the number of vessels formed; however, they were smaller in size. Vessel morphology and signalling pathways were also investigated using the mouse aortic ring assay, with exposures of 0.0, 0.5, 1.0, 5.0, or 10.0 μM CdCl2. Samples exposed to 10 μM experienced a significant increase in vessel length. However, no significant differences in phosphorylated PTEN and AKT were observed. The results of this study suggest that low levels of cadmium may disrupt angiogenesis, particularly the development of the embryonic vasculature in the yolk sac.
Author Keywords: Angiogenesis, Cadmium, Embryonic Development, Teratogenicity, Vascular Development, Vasculogenesis
Animal-mediated elemental cycling across time, space, and animal functional traits
Animals are essential to freshwater biogeochemistry and productivity. Through their excretion, aquatic consumers release bioavailable nutrients and carbon that can vary with animal taxonomic rank, trophic position, and abiotic factors such as light and nutrient supply. In fresh waters, light and nutrient supply is often modulated by dissolved organic matter (DOM), a "murky" component in the water that gives it a brown color and that may indirectly affect animal nutrient and carbon excretion. Additionally, contaminants can impact animal physiology, altering metabolism and inducing stress, further affecting nutrient and contaminant excretion. The size and structure of the ecosystem, including community composition and biomass, can also impact the contribution of aquatic animals to the elemental pool. To understand these dynamics, I examined animal‐mediated elemental cycling in freshwater ecosystems across gradients of DOM concentration and composition and under contaminant exposure. I tested fish and invertebrate nitrogen, phosphorus, and DOM excretion across trophic positions during two sampling events in Lake Erie and in naturally DOM-variable streams and lakes. I also investigated the effects of chronic exposure to silver nanoparticles (AgNP) under environmentally relevant conditions on fish nutrient and silver (Ag) release. I found that aquatic animals can be a substantial nutrient contributor to the nutrient pool, particularly when their population biomass is high and ambient nutrient concentrations are low. I also detected nonlinear relationships between animal nutrient excretion and DOM characteristics that varied with taxonomic rank and trophic position and that dampened at larger ecological scales. Importantly, I identified several fish DOM excretion signatures that differed relative to ambient DOM and reported the first fish Ag excretion rates under AgNPs exposure. My results underscore the context-dependency and variability inherent in animal-mediated elemental cycling, highlighting the critical role of animals as both modifiers and conduits of nutrients, DOM, and contaminants in aquatic ecosystems.
Author Keywords: carbon, consumer-nutrient driven dynamics, ecological stoichiometry, nitrogen, phosphorus, silver nanoparticles
Dissolved organic phosphorus and dissolved organic matter in Lake Erie and its tributaries
Phosphorus is the growth-limiting nutrient in freshwater environments. Dissolved organic phosphorus (DOP) refers to phosphorus within dissolved organic matter (DOM). Much of DOP is bioavailable, but it is poorly understood due to its complexity. This thesis explores the export DOP to Lake Erie from its tributaries, by investigating its speciation and concentration seasonally through weekly sampling of two tributaries and spatially across a river to lake transect. The rivermouth was a site of rapid transition, with lower concentrations of DOP in the lake than in the river and a greater proportion of P as DOP in the lake. Phosphomonoesters and aromatic DOM were coupled in the medium-sized Grand River, but not in the Upper Great Lakes-influenced Detroit River. Phosphodiesters and highly processed DOM were coupled in the Detroit River, but only during periods of low terrestrial inputs. Finally, we found that DOP is a large contributor to tributary phosphorus exports.
Author Keywords: dissolved organic matter, dissolved organic phosphorus, enzymatic hydrolysis, Lake Erie, nutrient export, rivermouth
The Depth of Death: Investigating the Mortuary Pattern of an Ancient Maya Chultun
The ancient Maya utilized aspects of their landscape within their religious rituals and ceremonies, including caves. The study of ritual cave use is known as Maya cave archaeology and archaeologists in this subfield suggest that all holes in the earth, be it natural or man-made were viewed as ritually significant spaces to the ancient Maya (Brady and Layco 2018). This thesis analyzes the mortuary pattern of a chultun in relation to natural cave rituals and/or burials to determine if both types of subterranean spaces functioned in ritually similar ways. Through excavation of a dual-chambered chultun at the site of Ka'kabish, this research established a parallel pattern between burials found in natural caves and the burials within a chultun/artificial cave. In so doing, this thesis provides much needed data in support of applying Maya cave archaeology theories and practices in the excavation and study of ancient Maya chultuns.
Author Keywords: Belize, cave burials, chultuns, Maya archaeology, Maya cave archaeology, mortuary archaeology
Range Expansion of Invasive Hybrid Cattails (Typha × Glauca) in the Prairie Pothole Region
Wetlands are highly susceptible to the invasion of invasive species. The invasive hybrid cattail (Typha × glauca) is prevalent in the southeastern Prairie Pothole Region (PPR) wetlands. However, concerns arise about its recent proliferation in the northwestern PPR without maternal T. angustifolia. To determine taxonomic distribution, I used species-specific PCR-RFLP and microsatellite markers for genotyping 245 samples from 50 northwest PPR sites. I found 75% T. latifolia, 7% T. angustifolia, 16% T. × glauca, and 2% backcrossed or advanced-generation hybrids. F1 T. × glauca has expanded in western PPR without its mother species, and the low occurrence of later-generation hybrids indicates their recent range expansion. Additionally, T. angustifolia offspring make fewer hybrids, which suggests that reproductive barriers may limit hybridization between parental species. This study highlights the vulnerability of prairies to cryptic invasions by Typha hybrids, and early detection of invasive species is a critical factor in wetland management success.
The Ethereal Path to Well-Being: An Exploration of the Connections Between Meditation, Spirituality, and Psychological Health
The traditions of spirituality and meditation have been found to connect to psychological health in the form of increased happiness, empathy, and decreased anxiety. The present study aimed to better understand how these practices might connect to such beneficial outcomes. A sample of 363 undergraduate student participants completed a questionnaire that measured their meditation practice, mindfulness, spirituality, happiness, empathy, and anxiety. Contrary to expectations, meditators and non- meditators did not significantly differ in their psychological health outcomes. These findings have implications for how meditators and non-meditator groups should be differentiated in research. Regarding spirituality, the purpose and meaning and innerness dimensions of the construct significantly predicted happiness and decreased anxiety, while the unified interconnectedness dimension significantly predicted empathy. The transcendence dimension of spirituality did not significantly predict psychological health. This pattern of results has implications for spiritual care interventions that intend to augment psychological health.
Author Keywords: Anxiety, Empathy, Happiness, Meditation, Psychological health, Spirituality
Navigating Erasure: Exploring the Limits and Potential of Indigenous Studies within the Settler Colonial Academy through Haudenosaunee Critical Self Reflexivity
This thesis explores the double-bind Indigenous Peoples encounter when pursuing post-secondary education in the field of Indigenous Studies. I argue that Indigenous voices deemed tolerable are incorporated into the commodification of Indigenous thought and experience for the Settler audiences who profit most from post-secondary institutions. My analysis discusses the possibilities for Indigenous Studies to navigate this parasitic relationship and assist Indigenous lives that academia renders unrecognizable. I examine my educational journey and conduct a literature review of the role that Settler Colonialism plays within Indigenous Studies. Through the use of critical self-reflexivity, this thesis employs Haudenosaunee political thought and Indigenous storywork to tell my personal narrative navigating the macro and micro dynamics within the academy that exploits Indigenous student's self-interest to maintain the Settler-Colonial status quo in higher education. I identify strategies to assist academics in conducting ethical research within Indigenous Studies and imagine insurgent education within the Canadian university.
Author Keywords: Commodification, Critical Indigenous Studies, Haudenosaunee, Settler Colonialism
The Interrelationships Between Sexual Agency, Sexual Consent Communication, Sexual Motivations, and Positive Sexual Evaluations
Sexual agency is receiving more attention in sexuality research though its contribution to sexual well-being has yet to be determined. Sexual agency has been theorized as an overarching concept comprised of both internal and external components. Both feelings and behaviours in the sexual domain can be impacted by prevalent culturally prescribed sexual scripts. The present study assessed sexual assertiveness, sexual self-concept, and comfort in sexual communication (i.e., together conceptualized as sexual agency) to determine if greater levels of these indices led to more direct consent communication, more intrinsic motivations for engaging in sex, and more positive sexual evaluations. Analyses were run separately on two samples: a student and community participant pool. Results indicate that greater sexual agency predicts being more intrinsically driven to engage in sex, using more direct consent communication, and reporting more positive evaluations of one's most recent sexual encounter. Gendered analyses reveal that cismen and ciswomen have similar levels of sexual agency, and rate their encounters similarly. Having greater sexual agency was a better predictor of experiencing sexual well-being compared to gender. Implications for policy development and sexual education curriculum reform are discussed, and future research directions are suggested.
Author Keywords: Sexual Agency, Sexual Consent, Sexual Motivations, Sexual Regret, Sexual Well-being