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Ploidy and Fitness Levels of Big Bluestem (Andropogon gerardii) Populations in South-Central Ontario: Implications for Seed Quality and Restoration
Ecological restoration of grassland ecosystems is increasing in scope in Ontario, as a result the demand for genetically appropriate, high-quality seeds of native plants is also increasing. This mixed methods study characterized fitness and seed quality traits using genetic, demographic and growth trial data for a keystone tallgrass prairie species Big bluestem (Andropogon gerardii). To estimate the ploidy levels in Big bluestem, our flow cytometric results indicated an average of 6.32 picograms of nuclear DNA within sixteen populations surveyed showing that hexaploid (6x) cytotypes are dominant in Southern and Central Ontario populations, aside from one 9x occurrence in Norfolk county, ON. Seed quality, measured through germination and viability testing did not change based on whether a population was remnant or restored. Concerningly, our study shows that remnant populations of Big bluestem are at risk of being lost as high quality seed sources likely because of the absence of stewardship and the resulting loss in population fitness. Workshops with prairie restoration practitioners suggest that there is significant vision behind the future of this work in the province, and that an ecosystem wide seed strategy for keystone tallgrass prairie species is a necessary next step to increase the sustainability of seed-based restoration strategies and preserve remnant site genetics.
Author Keywords: Big bluestem (Andropogon gerardii), Ecological Restoration, fitness, polyploidy, practitioner, seed quality
Bayesian Network Model of Mercury Exposure to Aquatic Ecosystems of the Mackenzie Watershed
A significant portion (15-20%) of mercury (Hg) in the Arctic Ocean is believed to originate from Arctic rivers, such as the Mackenzie River watershed in the NWT. Recent (2005- 2020) Hg monitoring data of freshwater and fish tissue and environmental model outputs were compiled and used to develop a Bayesian Network Relative Risk model (BN-RRM), a probabilistic model capable of analyzing causal relationships. The objectives of the model were to estimate the risk posed to fish health and the subsequent dietary Hg-exposure to humans; to compare the relative risks between regions of the watershed; and to identify the influential Hg sources. The output of the BN-RRMs differed significantly throughout the watershed, with atmospheric Hg deposition and soil erosion Hg release consistently flagged as important explanatory variables. Analysis of the endpoint uncertainties revealed gaps in knowledge and in Hg datasets, which should be the focus of study for future monitoring programs.
Author Keywords: Aquatic Ecosystems, Arctic, Bayesian Network, Mercury, Risk Assessment, Toxicology
Fractionation and Evaluation of Carapa guianensis Seed Oil
The seed oil of Carapa guianensis, known as Crabwood oil (CWO) and its fractionation was studied, focussing on chemical composition and physicochemical functionality. Compounds never reported before in CWO were identified, including limonoids such as Trichillin, ketostearic and hydroxyoleic acids. Yield of olein and stearin fractions correlated strongly with solvent polarity. Important bioactives were partitioned as a function of solvent polarity in the olein vs. stearin fractions. For example, up to 35% more of Gedunin were present in the liquid fractions. Solid fat content, crystallization and melting temperature and microstructure were significantly affected by fractionation and shown to be strongly correlated with solvent polarity. Fractionation was demonstrated to be a powerful tool to extend the physical functionality of CWO and concentrate its bioactives.
Author Keywords: Carapa guianensis, Chemical characteristics, Crabwood Oil, Dry and Solvent fractionation, Fractionation, Physical characteristics
It Takes a Village: Cooperation and Relationships Between Local ENGOs and Municipal Governments for Environmental Initiatives
Local environmental initiatives can create visible and essential changes and inspire greater environmental action. Municipal governments and local environmental non-governmental organizations (ENGOs) are important local actors, but their partnerships and relationships have not received much attention. This thesis examines what activities and relationships have been developed between municipal governments and ENGOs in the Peterborough region, what benefits they gain and what challenges they face during collaboration, and how these partnerships affect public perceptions of the organizations. I conducted 14 interviews with members of local ENGOs and municipalities and received 52 survey responses from residents. The findings indicate groups have unique relationships for planning, programming, and advocacy activities. Relationships were key and challenges included lack of time and prioritization, communication, and public buy-in. Partnerships provide an opportunity to share positive accomplishments and build reputation. This study sheds light on the complex relationships among local organizations and provides recommendations for improving partnerships.
Author Keywords: Community Engagement, Environmental Non-Governmental Organizations, Local Sustainability, Municipal Government, Partnerships, Relationships
Automated Grading of UML Use Case Diagrams
This thesis presents an approach for automated grading of UML Use Case diagrams. Many software engineering courses require students to learn how to model the behavioural features of a problem domain or an object-oriented design in the form of a use case diagram. Because assessing UML assignments is a time-consuming and labor-intensive operation, there is a need for an automated grading strategy that may help instructors by speeding up the grading process while also maintaining uniformity and fairness in large classrooms. The effectiveness of this automated grading approach was assessed by applying it to two real-world assignments. We demonstrate how the result is similar to manual grading, which was less than 7% on average; and when we applied some strategies, such as configuring settings and using multiple solutions, the average differences were even lower. Also, the grading methods and the tool are proposed and empirically validated.
Author Keywords: Automated Grading, Compare Models, Use Case
Labour, Learning, and Leisure: The Technical Culture of Practice in Video Game Live Streaming
Games, and especially video games are fast becoming the most pervasive media form, and live streaming games is fast becoming the most pervasive way of experiencing those games. This thesis looks at the history of broadcast, the practices of technological hobbyists, the social and technological aspect of games, gaming communities that transform game narratives, and gaming communities that transform political narratives. It demonstrates how the study of video game live streaming can be used as a model to study and analyze the production, consumption, and reciprocal relationship between the producers and consumers of media.
Author Keywords: Bill Gates is the Devil, Broadcast, Gaming, Ham Radio, Live Streaming, Video Game Live Streaming
Seeds: More than Food
This study used literature from the social and ecological sciences to look at the practice of seed saving in the Global North for adaptation in the face of climate change. The hypothesis is that small-scale gardeners and farmers are adapting seeds to the changing climate as they grow them, which is not the case for growers who grow only food. However seed savers face obstacles and are dwindling in number – even though the practice is thousands of years old. Using survey and interviews of seed savers in two communities in Ontario Canada (one Indigenous and one settler), this mixed methods Participatory Action Research project explores the challenges and opportunities for seed saving in the context of climate change. The results indicate that both seed savers and their crops are adapting to climate change, but that the challenges and implications diverge for Indigenous vs settler communities. A framework of recommendations to advance seed saving in a beneficial, decolonizing way is proposed.
Author Keywords: climate change, Food security, food sovereignty, Seed Keeping, Seed Saving, Sustainable Food Systems
Our 'Canada': National Narratives and the Dangers of Bourgeois Mythologies and Hegemonic Canadian Propaganda
This thesis argues that Canada, as it is regarded by the Canadian citizenry, exists as a collection of public-facing narratives within a collectively imagined national mythos. This mythos, as it stands in 2022, is an accumulation of layers of narratives built on the foundation of former British imperial myths honed by bourgeois ideologies and ideals into a uniquely 'Canadian' nationalism through the propaganda of the Great War, the Second World War, the Cold War, and the 'War on Terror.' In attempting to deconstruct this collection of narratives, this thesis employs a historical materialist approach and uses the theories of Marx, Lenin, Gramsci, and Althusser to argue for the importance of an internationalist perspective which has been neglected in the insistence on an inward domestic approach to the identity of Canada as a nation.
Author Keywords: Canada, Capitalism, Marxism, Media, Neoimperialism, Propaganda
Navigating Uncertainty: Exploring Parents Knowledge of Concussion Management and Neuropsychological Baseline Testing
Past research on parents' knowledge of concussion has shown that they understand the severity and consequences associated with the injury but have existing gaps in their knowledge for its management. This is a cause for concern due to the critical role parents play in their child's management process. This present study sought to address this area of concern with semi- structured focus groups to gain an insight on parents' perceptions and experiences with concussion management and an alternative method of care - neuropsychological baseline testing. What emerged from the focus groups was the core theme of navigating uncertainty. This theme encompassed the participants perceptions and experiences with having to take on the role of managing their child's concussion without a complete understanding of the management process. It incorporated aspects of injury diagnosis, methods of management, communication among stakeholders within the sporting community, the influence of concussion policy and parents' perceptions of neuropsychological baseline testing in the management process. A model was constructed to display the factors that influenced and guided parents' engagement with neuropsychological baseline testing.
Author Keywords: Communication, Concussion, Diagnosis, Management, Neuropsychological Baseline Testing, Uncertainty
Camelids on the Coast? Investigating Trade and Early Camelid Herding through Stable Isotope Analysis of Formative to Late Intermediate Period Textiles from the Atacama Desert, Chile
This research presents stable carbon and nitrogen isotope data of camelid fiber (n=238) from the coast of Arica, the Lluta Valley, and Azapa Valley – all located in the hyper-arid core of the Atacama Desert in northern Chile. Samples included are attributed to the Formative (1000 BCE– 200 CE), Middle (200 CE – 1000CE), and Late Intermediate (1000 CE – 1450 CE) periods. The principal aim of this research was to determine whether camelid fiber uncovered at archaeological sites on the coast were likely obtained from locally managed camelid herds, or whether fiber was traded from the highlands. The majority of samples are consistent with a highland origin; however, a small group of samples is consistent with having come from animals herded outside the highlands, possibly locally in the coastal river valleys. There was also an increase in fiber δ13C between the Formative and Late Intermediate Periods, indicating an increased reliance on maize and agricultural intensification between these periods.
Author Keywords: Camelid, Carbon, Chile, Keratin, Nitrogen, Stable isotope analysis